Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SOI80_RS16655 Genome accession   NZ_CP139270
Coordinates   3419694..3420494 (-) Length   266 a.a.
NCBI ID   WP_181949727.1    Uniprot ID   -
Organism   Acinetobacter pittii strain HCG62     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3414694..3425494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI80_RS16635 (SOI80_16640) - 3415759..3416457 (-) 699 WP_017398816.1 YfgM family protein -
  SOI80_RS16640 (SOI80_16645) hisS 3416486..3417778 (-) 1293 WP_032042050.1 histidine--tRNA ligase -
  SOI80_RS16645 (SOI80_16650) ispG 3417775..3418890 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SOI80_RS16650 (SOI80_16655) - 3418912..3419703 (-) 792 WP_032042051.1 helix-turn-helix domain-containing protein -
  SOI80_RS16655 (SOI80_16660) pilF 3419694..3420494 (-) 801 WP_181949727.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SOI80_RS16660 (SOI80_16665) rlmN 3420509..3421744 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SOI80_RS16665 (SOI80_16670) ndk 3421868..3422299 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  SOI80_RS16670 (SOI80_16675) iscX 3422413..3422613 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  SOI80_RS16675 (SOI80_16680) - 3422713..3423264 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  SOI80_RS16680 (SOI80_16685) - 3423289..3424578 (-) 1290 WP_075383343.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30116.35 Da        Isoelectric Point: 9.0417

>NTDB_id=908578 SOI80_RS16655 WP_181949727.1 3419694..3420494(-) (pilF) [Acinetobacter pittii strain HCG62]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
TNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=908578 SOI80_RS16655 WP_181949727.1 3419694..3420494(-) (pilF) [Acinetobacter pittii strain HCG62]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ATTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCAGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
ACGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

75.769

97.744

0.741