Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SOI79_RS04900 Genome accession   NZ_CP139266
Coordinates   1025836..1026636 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain HUM14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020836..1031636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI79_RS04880 (SOI79_04880) - 1021901..1022599 (-) 699 WP_002114884.1 YfgM family protein -
  SOI79_RS04885 (SOI79_04885) hisS 1022628..1023920 (-) 1293 WP_068976567.1 histidine--tRNA ligase -
  SOI79_RS04890 (SOI79_04890) ispG 1023917..1025032 (-) 1116 WP_068976568.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SOI79_RS04895 (SOI79_04895) - 1025054..1025845 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  SOI79_RS04900 (SOI79_04900) pilF 1025836..1026636 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SOI79_RS04905 (SOI79_04905) rlmN 1026651..1027886 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SOI79_RS04910 (SOI79_04910) ndk 1028010..1028441 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  SOI79_RS04915 (SOI79_04915) iscX 1028555..1028755 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  SOI79_RS04920 (SOI79_04920) - 1028855..1029406 (-) 552 WP_017482038.1 phosphatase PAP2 family protein -
  SOI79_RS04925 (SOI79_04925) - 1029431..1030720 (-) 1290 WP_081305971.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=908508 SOI79_RS04900 WP_002114699.1 1025836..1026636(-) (pilF) [Acinetobacter pittii strain HUM14]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=908508 SOI79_RS04900 WP_002114699.1 1025836..1026636(-) (pilF) [Acinetobacter pittii strain HUM14]
TTGAGTATTCCAAAGTTAAAAACGGTTTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCTGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744