Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SOI70_RS14195 Genome accession   NZ_CP139258
Coordinates   2953552..2954352 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain HUM3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2948552..2959352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI70_RS14175 (SOI70_14195) - 2949617..2950315 (-) 699 WP_002114884.1 YfgM family protein -
  SOI70_RS14180 (SOI70_14200) hisS 2950344..2951636 (-) 1293 WP_002114994.1 histidine--tRNA ligase -
  SOI70_RS14185 (SOI70_14205) ispG 2951633..2952748 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SOI70_RS14190 (SOI70_14210) - 2952770..2953561 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  SOI70_RS14195 (SOI70_14215) pilF 2953552..2954352 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SOI70_RS14200 (SOI70_14220) rlmN 2954367..2955602 (-) 1236 WP_005079127.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SOI70_RS14205 (SOI70_14225) ndk 2955726..2956157 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  SOI70_RS14210 (SOI70_14230) iscX 2956271..2956471 (-) 201 WP_002115051.1 Fe-S cluster assembly protein IscX -
  SOI70_RS14215 (SOI70_14235) - 2956571..2957122 (-) 552 WP_005079131.1 phosphatase PAP2 family protein -
  SOI70_RS14220 (SOI70_14240) - 2957147..2958436 (-) 1290 WP_080632755.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=908438 SOI70_RS14195 WP_002114699.1 2953552..2954352(-) (pilF) [Acinetobacter pittii strain HUM3]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=908438 SOI70_RS14195 WP_002114699.1 2953552..2954352(-) (pilF) [Acinetobacter pittii strain HUM3]
TTGAGTATTCCAAAGTTAAAAACGGTTTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCTGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744