Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SOI71_RS15935 Genome accession   NZ_CP139251
Coordinates   3349882..3350682 (-) Length   266 a.a.
NCBI ID   WP_205668704.1    Uniprot ID   -
Organism   Acinetobacter pittii strain MCR53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3344882..3355682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI71_RS15915 (SOI71_15905) - 3345947..3346645 (-) 699 WP_017398816.1 YfgM family protein -
  SOI71_RS15920 (SOI71_15910) hisS 3346674..3347966 (-) 1293 WP_017385736.1 histidine--tRNA ligase -
  SOI71_RS15925 (SOI71_15915) ispG 3347963..3349078 (-) 1116 WP_033849427.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SOI71_RS15930 (SOI71_15920) - 3349100..3349891 (-) 792 WP_033849429.1 helix-turn-helix domain-containing protein -
  SOI71_RS15935 (SOI71_15925) pilF 3349882..3350682 (-) 801 WP_205668704.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SOI71_RS15940 (SOI71_15930) rlmN 3350697..3351932 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SOI71_RS15945 (SOI71_15935) ndk 3352056..3352487 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  SOI71_RS15950 (SOI71_15940) iscX 3352601..3352801 (-) 201 WP_002115051.1 Fe-S cluster assembly protein IscX -
  SOI71_RS15955 (SOI71_15945) - 3352901..3353452 (-) 552 WP_290012767.1 phosphatase PAP2 family protein -
  SOI71_RS15960 (SOI71_15950) - 3353477..3354766 (-) 1290 WP_104075006.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30026.29 Da        Isoelectric Point: 8.8665

>NTDB_id=908343 SOI71_RS15935 WP_205668704.1 3349882..3350682(-) (pilF) [Acinetobacter pittii strain MCR53]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRICLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=908343 SOI71_RS15935 WP_205668704.1 3349882..3350682(-) (pilF) [Acinetobacter pittii strain MCR53]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTCAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTGTTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

75.769

97.744

0.741