Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SOI76_RS16020 Genome accession   NZ_CP139249
Coordinates   3340044..3340844 (-) Length   266 a.a.
NCBI ID   WP_205668465.1    Uniprot ID   -
Organism   Acinetobacter pittii strain MCR8900     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3335044..3345844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI76_RS16000 (SOI76_16000) - 3336109..3336807 (-) 699 WP_104080353.1 YfgM family protein -
  SOI76_RS16005 (SOI76_16005) hisS 3336836..3338128 (-) 1293 WP_104080352.1 histidine--tRNA ligase -
  SOI76_RS16010 (SOI76_16010) ispG 3338125..3339240 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SOI76_RS16015 (SOI76_16015) - 3339262..3340053 (-) 792 WP_016142242.1 helix-turn-helix domain-containing protein -
  SOI76_RS16020 (SOI76_16020) pilF 3340044..3340844 (-) 801 WP_205668465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SOI76_RS16025 (SOI76_16025) rlmN 3340859..3342094 (-) 1236 WP_104080350.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SOI76_RS16030 (SOI76_16030) ndk 3342218..3342649 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  SOI76_RS16035 (SOI76_16035) iscX 3342763..3342963 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  SOI76_RS16040 (SOI76_16040) - 3343063..3343614 (-) 552 WP_032054389.1 phosphatase PAP2 family protein -
  SOI76_RS16045 (SOI76_16045) - 3343639..3344925 (-) 1287 WP_104080349.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30068.25 Da        Isoelectric Point: 9.0424

>NTDB_id=908302 SOI76_RS16020 WP_205668465.1 3340044..3340844(-) (pilF) [Acinetobacter pittii strain MCR8900]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKTNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNEAIEQFRIAGATLGYNQRYQALENLGRIYLKLGDV
VNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVHTVGQKNQGARALWIGVRVARANADKMGTQALVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=908302 SOI76_RS16020 WP_205668465.1 3340044..3340844(-) (pilF) [Acinetobacter pittii strain MCR8900]
TTGAGTATTCCAAAGTTGAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAAAAAGCAGTAAAAGTTCGTACACAACTTGCTGCTGAACATATCCGTTCTGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTGGATAGCCGCGATGCAACGGCAAACATGATGATGGGGATTTTG
CTACAGCAAGAAGGTAGCAAAACCAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCGTCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGAGCGTTATAATGAGGCGATTGAGCAATTCCGTATTG
CTGGAGCAACATTAGGTTATAACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GTGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCATACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAACGCAAGCGTTAGTAAACCAA
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-205)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

92.105

100

0.921
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726