Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SOI69_RS12640 Genome accession   NZ_CP139248
Coordinates   2691127..2691927 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain HCG18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2686127..2696927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI69_RS12620 (SOI69_12620) - 2687192..2687890 (-) 699 WP_017398816.1 YfgM family protein -
  SOI69_RS12625 (SOI69_12625) hisS 2687919..2689211 (-) 1293 WP_032005186.1 histidine--tRNA ligase -
  SOI69_RS12630 (SOI69_12630) ispG 2689208..2690323 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SOI69_RS12635 (SOI69_12635) - 2690345..2691136 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  SOI69_RS12640 (SOI69_12640) pilF 2691127..2691927 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SOI69_RS12645 (SOI69_12645) rlmN 2691942..2693177 (-) 1236 WP_125825125.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SOI69_RS12650 (SOI69_12650) ndk 2693301..2693732 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  SOI69_RS12655 (SOI69_12655) iscX 2693846..2694046 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  SOI69_RS12660 (SOI69_12660) - 2694146..2694697 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  SOI69_RS12665 (SOI69_12665) - 2694722..2696011 (-) 1290 WP_320540546.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=908240 SOI69_RS12640 WP_002114699.1 2691127..2691927(-) (pilF) [Acinetobacter pittii strain HCG18]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=908240 SOI69_RS12640 WP_002114699.1 2691127..2691927(-) (pilF) [Acinetobacter pittii strain HCG18]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGCTGCCAGACAACGCA
TACGCAAAAAAAAGATCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGTCGTGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744