Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABK32_RS11420 Genome accession   NZ_AP027829
Coordinates   2314252..2314929 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 2017.17.03CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2309252..2319929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABK32_RS11385 (VEE61_21940) gspG 2309732..2310169 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  AABK32_RS11390 (VEE61_21950) gspH 2310177..2310686 (+) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  AABK32_RS11395 (VEE61_21960) gspI 2310683..2311060 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABK32_RS11400 (VEE61_21970) gspJ 2311053..2311640 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABK32_RS11405 (VEE61_21980) gspK 2311633..2312616 (+) 984 WP_089078830.1 type II secretion system minor pseudopilin GspK -
  AABK32_RS11410 (VEE61_21990) gspL 2312631..2313794 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  AABK32_RS11415 (VEE61_22000) gspM 2313791..2314252 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  AABK32_RS11420 (VEE61_22010) pilD 2314252..2314929 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  AABK32_RS11425 (VEE61_22020) bfr 2314958..2315434 (-) 477 WP_000675504.1 bacterioferritin -
  AABK32_RS11430 (VEE61_22030) bfd 2315506..2315700 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABK32_RS11435 (VEE61_22040) chiA 2315869..2318562 (-) 2694 Protein_2232 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=90789 AABK32_RS11420 WP_000178161.1 2314252..2314929(+) (pilD) [Escherichia coli strain 2017.17.03CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=90789 AABK32_RS11420 WP_000178161.1 2314252..2314929(+) (pilD) [Escherichia coli strain 2017.17.03CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378