Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   SIS06_RS14090 Genome accession   NZ_CP139188
Coordinates   2655315..2655725 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain H17-16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2650315..2660725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIS06_RS14060 (SIS06_14060) - 2650401..2650541 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  SIS06_RS14065 (SIS06_14065) yqeF 2650847..2651578 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  SIS06_RS14070 (SIS06_14070) cwlH 2651830..2652582 (-) 753 WP_072174288.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  SIS06_RS14075 (SIS06_14075) yqeD 2652769..2653395 (+) 627 WP_161619430.1 TVP38/TMEM64 family protein -
  SIS06_RS14080 (SIS06_14080) gnd 2653415..2654308 (-) 894 WP_070547557.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  SIS06_RS14085 (SIS06_14085) yqeB 2654560..2655282 (+) 723 WP_049141193.1 hypothetical protein -
  SIS06_RS14090 (SIS06_14090) nucA/comI 2655315..2655725 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  SIS06_RS14095 (SIS06_14095) - 2655921..2656271 (+) 351 Protein_2732 sigma-70 family RNA polymerase sigma factor -
  SIS06_RS14100 (SIS06_14100) spoIVCA 2656299..2657759 (-) 1461 WP_283934478.1 site-specific DNA recombinase SpoIVCA -
  SIS06_RS14105 (SIS06_14105) - 2657717..2657895 (-) 179 Protein_2734 hypothetical protein -
  SIS06_RS14110 (SIS06_14110) - 2658328..2658921 (-) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  SIS06_RS14115 (SIS06_14115) - 2659079..2660074 (+) 996 WP_029318099.1 NADPH:quinone oxidoreductase family protein -
  SIS06_RS14120 (SIS06_14120) - 2660402..2660635 (+) 234 Protein_2737 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=907645 SIS06_RS14090 WP_009967785.1 2655315..2655725(-) (nucA/comI) [Bacillus subtilis strain H17-16]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=907645 SIS06_RS14090 WP_009967785.1 2655315..2655725(-) (nucA/comI) [Bacillus subtilis strain H17-16]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACTGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529