Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PTKU15_RS01585 Genome accession   NZ_AP025256
Coordinates   345680..347200 (-) Length   506 a.a.
NCBI ID   WP_042306308.1    Uniprot ID   A0A2I8EFQ9
Organism   Paraburkholderia terrae strain KU-15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 340680..352200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTKU15_RS01560 (PTKU15_03070) - 341459..342094 (-) 636 WP_042306311.1 LON peptidase substrate-binding domain-containing protein -
  PTKU15_RS01565 (PTKU15_03080) rapZ 342159..343052 (-) 894 WP_042306310.1 RNase adapter RapZ -
  PTKU15_RS01570 (PTKU15_03090) hprK 343130..344098 (-) 969 WP_007579699.1 HPr(Ser) kinase/phosphatase -
  PTKU15_RS01575 (PTKU15_03100) - 344329..344844 (-) 516 WP_248556052.1 PTS sugar transporter subunit IIA -
  PTKU15_RS01580 (PTKU15_03110) hpf 345145..345504 (-) 360 WP_007579701.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PTKU15_RS01585 (PTKU15_03120) rpoN/rpoN1 345680..347200 (-) 1521 WP_042306308.1 RNA polymerase factor sigma-54 Machinery gene
  PTKU15_RS01590 (PTKU15_03130) lptB 347430..348212 (-) 783 WP_042306307.1 LPS export ABC transporter ATP-binding protein -
  PTKU15_RS01595 (PTKU15_03140) lptA 348209..348886 (-) 678 WP_091777781.1 lipopolysaccharide transport periplasmic protein LptA -
  PTKU15_RS01600 (PTKU15_03150) lptC 348908..349513 (-) 606 WP_042306305.1 LPS export ABC transporter periplasmic protein LptC -
  PTKU15_RS01605 (PTKU15_03160) - 349510..350070 (-) 561 WP_248555312.1 KdsC family phosphatase -
  PTKU15_RS01610 (PTKU15_03170) kdsD 350070..351053 (-) 984 WP_007579707.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55681.53 Da        Isoelectric Point: 4.8796

>NTDB_id=90719 PTKU15_RS01585 WP_042306308.1 345680..347200(-) (rpoN/rpoN1) [Paraburkholderia terrae strain KU-15]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVSMAISQNPLLETEDDWIASPLRVAADGTVISQTSQNTSPEGMS
SSPPSSSSSTSSGESQENGEPQGVDEYNGMGSDSNSDANQWNLDDYGRSGNASDDDDLPPLQIHESTTTLRDHLTAQLRV
TGASARDRALITFLIESLDDDGYLTATFDEILTDLPEELEVDTDELNAALALLHSFDPAGVGARSASECLRLQLCRLEPS
PTRTLALDIVAHHLELLAARDFTRLRKYLKASDDDLREAHALIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELN
PEVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREI
ADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQG
FVVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=90719 PTKU15_RS01585 WP_042306308.1 345680..347200(-) (rpoN/rpoN1) [Paraburkholderia terrae strain KU-15]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTTGCGCTGACACCACAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCTACGCTCGAACTGCAGCAGGAAGTGTCGATGGCGATCTCGCAGAATCCGTTGCTCGAAACGGAAGACGACTGGA
TCGCCAGTCCGTTGCGCGTCGCCGCCGACGGCACGGTCATCTCGCAGACGTCGCAGAACACCTCGCCGGAAGGAATGTCG
AGTTCGCCGCCGTCTTCTTCGTCGTCCACTTCCTCCGGCGAAAGCCAGGAGAACGGCGAACCCCAGGGCGTCGACGAATA
CAACGGCATGGGTTCGGACTCGAACTCGGATGCCAACCAGTGGAATCTCGACGACTACGGCCGTTCGGGCAACGCATCGG
ACGACGACGATCTACCGCCGCTGCAAATCCACGAATCGACCACGACGCTGCGCGATCACTTGACTGCTCAACTTCGTGTC
ACGGGAGCAAGCGCACGCGATCGCGCGCTCATCACGTTCCTGATCGAATCACTCGACGACGACGGCTATCTGACTGCCAC
CTTCGACGAAATCCTCACCGATCTCCCCGAAGAACTGGAAGTCGATACCGACGAACTCAACGCGGCCCTCGCCTTACTGC
ACAGCTTCGACCCAGCGGGTGTCGGCGCGCGTTCGGCGTCCGAATGCCTGCGTTTGCAGTTGTGCCGGCTCGAGCCGTCG
CCCACGCGCACGCTCGCGCTGGACATCGTCGCGCATCATCTGGAACTGCTCGCCGCGCGCGACTTCACGCGTCTGCGCAA
ATACCTGAAGGCAAGCGACGACGATCTGCGCGAAGCGCATGCGCTGATCCGCTCGCTGGAGCCATTCCCCGGCGCGGCCT
ACGGTAAGGCCGAAGCGGACTACGTCGTGCCCGACATCATGGTGCGCAAAACGGCACAGGGATGGCAGGCGGAACTGAAT
CCCGAAGTCGTGCCGAAACTGCGTATCAACCATCTGTACGCGAATATCCTGCGCAACAATCGCGGCGATCCGGGCAGTGG
TTCGCTGCGGCAGCAACTGCAGGAAGCGCGTTGGCTGATCAAGAATATCCAGCAGCGGTTCGAGACGATCCTGCGTGTCG
CACAGGCAATTGTGGAGCGTCAAAAGAACTTTTTCGCCCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATA
GCTGATACGCTGGGTTTACACGAGTCGACGGTCTCACGTGTGACAACCGGCAAGTACATGCTCACCCCATTCGGGACGCT
TGAATTTAAGTACTTCTTCGGATCGCACGTATCCACCGACACGGGGGGCGCGGCCTCTTCAACGGCCATTCGCGCACTCA
TCAAGCAACTGATAGGAGCGGAAGACACCAAATCTCCTCTTTCAGACAGCCGCATTGCCGAACTGCTGGCGGAACAGGGT
TTCGTCGTCGCTCGCCGTACCGTTGCGAAATACCGCGAAGCACTGAAGATCCCAGCAGTCAATCTGCGCAAGTCTCTTTA
G

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(349-504)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-333)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I8EFQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.601

100

0.67

Multiple sequence alignment