Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABMW40_RS24640 Genome accession   NZ_CP158049
Coordinates   5269821..5271314 (+) Length   497 a.a.
NCBI ID   WP_023107818.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2-216     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5264821..5276314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABMW40_RS24610 (ABMW40_24600) - 5265033..5265842 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  ABMW40_RS24615 (ABMW40_24605) kdsD 5266256..5267236 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  ABMW40_RS24620 (ABMW40_24610) - 5267236..5267775 (+) 540 WP_003098849.1 HAD family hydrolase -
  ABMW40_RS24625 (ABMW40_24615) lptC 5267784..5268356 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  ABMW40_RS24630 (ABMW40_24620) lptA 5268343..5268870 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  ABMW40_RS24635 (ABMW40_24625) lptB 5268870..5269595 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  ABMW40_RS24640 (ABMW40_24630) rpoN/rpoN1 5269821..5271314 (+) 1494 WP_023107818.1 RNA polymerase factor sigma-54 Machinery gene
  ABMW40_RS24645 (ABMW40_24635) hpf 5271392..5271700 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ABMW40_RS24650 (ABMW40_24640) ptsN 5271714..5272178 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABMW40_RS24655 (ABMW40_24645) rapZ 5272180..5273040 (+) 861 WP_003094361.1 RNase adapter RapZ -
  ABMW40_RS24660 (ABMW40_24650) - 5273070..5273342 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  ABMW40_RS24665 (ABMW40_24655) - 5273439..5274371 (-) 933 WP_003120918.1 ZIP family metal transporter -
  ABMW40_RS24670 (ABMW40_24660) - 5274390..5275001 (-) 612 WP_003098862.1 superoxide dismutase -
  ABMW40_RS24675 (ABMW40_24665) - 5275014..5275463 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56028.18 Da        Isoelectric Point: 4.4327

>NTDB_id=907100 ABMW40_RS24640 WP_023107818.1 5269821..5271314(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 2-216]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTAWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=907100 ABMW40_RS24640 WP_023107818.1 5269821..5271314(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 2-216]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTACTGCTGCAACTGCGCCAGTTGCCGTCCACCACGGCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATTGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477