Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ82_RS01900 Genome accession   NZ_AP027822
Coordinates   412535..413212 (-) Length   225 a.a.
NCBI ID   WP_274441519.1    Uniprot ID   -
Organism   Escherichia coli strain 2017.17.01CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407535..418212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ82_RS01885 (VEE60_03800) chiA 408902..411595 (+) 2694 WP_338382933.1 bifunctional chitinase/lysozyme -
  AABJ82_RS01890 (VEE60_03810) bfd 411764..411958 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABJ82_RS01895 (VEE60_03820) bfr 412030..412506 (+) 477 WP_000675504.1 bacterioferritin -
  AABJ82_RS01900 (VEE60_03830) pilD 412535..413212 (-) 678 WP_274441519.1 prepilin peptidase GspO Machinery gene
  AABJ82_RS01905 (VEE60_03840) gspM 413212..413673 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AABJ82_RS01910 (VEE60_03850) gspL 413670..414833 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  AABJ82_RS01915 (VEE60_03860) gspK 414848..415831 (-) 984 WP_088550607.1 type II secretion system minor pseudopilin GspK -
  AABJ82_RS01920 (VEE60_03870) gspJ 415824..416411 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABJ82_RS01925 (VEE60_03880) gspI 416404..416781 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABJ82_RS01930 (VEE60_03890) gspH 416778..417287 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  AABJ82_RS01935 (VEE60_03900) gspG 417295..417732 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24873.73 Da        Isoelectric Point: 7.6354

>NTDB_id=90682 AABJ82_RS01900 WP_274441519.1 412535..413212(-) (pilD) [Escherichia coli strain 2017.17.01CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLSDILTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=90682 AABJ82_RS01900 WP_274441519.1 412535..413212(-) (pilD) [Escherichia coli strain 2017.17.01CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGTCCGACATACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.558

100

0.373