Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   IDSSA_RS00315 Genome accession   NZ_AP025249
Coordinates   68157..69503 (-) Length   448 a.a.
NCBI ID   WP_000816499.1    Uniprot ID   Q9R3W9
Organism   Staphylococcus aureus strain IDSA1     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 40878..69503 68157..69503 within 0


Gene organization within MGE regions


Location: 40878..69503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IDSSA_RS00165 (IDSSA_0100340) ccrC 40878..42563 (+) 1686 WP_000676600.1 cassette chromosome recombinase CcrC -
  IDSSA_RS00170 (IDSSA_0100350) tnpA 42611..43096 (+) 486 WP_000093392.1 IS200/IS605 family transposase -
  IDSSA_RS00175 (IDSSA_0100360) - 43388..43726 (+) 339 WP_000175892.1 SAUGI family uracil-DNA glycosylase inhibitor -
  IDSSA_RS00180 (IDSSA_0100370) - 43820..44131 (+) 312 WP_001166716.1 DUF960 family protein -
  IDSSA_RS00185 (IDSSA_0100380) - 44147..44653 (+) 507 WP_000848380.1 DUF1643 domain-containing protein -
  IDSSA_RS00190 (IDSSA_0100390) - 44674..44991 (+) 318 WP_000659046.1 JAB domain-containing protein -
  IDSSA_RS00195 (IDSSA_0100400) - 45183..45857 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  IDSSA_RS00200 - 45893..46282 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  IDSSA_RS00205 (IDSSA_0100420) - 47079..47822 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  IDSSA_RS00210 (IDSSA_0100430) - 47919..48347 (+) 429 WP_000872606.1 MaoC family dehydratase -
  IDSSA_RS00215 (IDSSA_0100440) mecA 48393..50402 (-) 2010 WP_078101914.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  IDSSA_RS00220 (IDSSA_0100450) mecR1 50499..52256 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  IDSSA_RS00225 mecI 52256..52627 (+) 372 Protein_42 mecA-type methicillin resistance repressor MecI -
  IDSSA_RS00230 psm-mec 52712..52780 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  IDSSA_RS00235 (IDSSA_0100470) - 53100..54248 (+) 1149 WP_000427807.1 ROK family transcriptional regulator -
  IDSSA_RS00240 (IDSSA_0100490) cstB 54362..55696 (-) 1335 Protein_45 persulfide dioxygenase-sulfurtransferase CstB -
  IDSSA_RS00245 (IDSSA_0100500) - 55728..56792 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  IDSSA_RS00250 (IDSSA_0100510) cstR 56928..57188 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  IDSSA_RS00255 (IDSSA_0100520) - 57188..57826 (+) 639 Protein_48 sulfite exporter TauE/SafE family protein -
  IDSSA_RS00260 (IDSSA_0100530) - 58100..58717 (-) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  IDSSA_RS00265 (IDSSA_0100540) - 58798..61212 (-) 2415 WP_000378485.1 cadmium-translocating P-type ATPase CadA -
  IDSSA_RS00270 (IDSSA_0100550) - 61205..61570 (-) 366 WP_000159127.1 metalloregulator ArsR/SmtB family transcription factor -
  IDSSA_RS00275 (IDSSA_0100560) - 61756..62133 (-) 378 WP_001041908.1 DUF6262 family protein -
  IDSSA_RS00280 (IDSSA_0100570) - 62140..64032 (-) 1893 WP_014532403.1 site-specific integrase -
  IDSSA_RS00285 (IDSSA_0100580) - 64227..64550 (-) 324 WP_000088091.1 JAB domain-containing protein -
  IDSSA_RS00290 (IDSSA_0100590) - 64543..64749 (-) 207 WP_001077283.1 hypothetical protein -
  IDSSA_RS00295 (IDSSA_0100600) - 64751..65272 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  IDSSA_RS00300 (IDSSA_0100610) - 65291..65602 (-) 312 WP_000833212.1 DUF960 family protein -
  IDSSA_RS00305 (IDSSA_0100620) - 65687..66037 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  IDSSA_RS00310 (IDSSA_0100630) ccrB 66508..68136 (-) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  IDSSA_RS00315 (IDSSA_0100640) ccrA 68157..69503 (-) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 52138.19 Da        Isoelectric Point: 10.1760

>NTDB_id=90673 IDSSA_RS00315 WP_000816499.1 68157..69503(-) (ccrA) [Staphylococcus aureus strain IDSA1]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=90673 IDSSA_RS00315 WP_000816499.1 68157..69503(-) (ccrA) [Staphylococcus aureus strain IDSA1]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(168-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9R3W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

78.174

100

0.783
  ccrA Staphylococcus aureus N315

72.383

100

0.725

Multiple sequence alignment