Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABL174_RS02900 Genome accession   NZ_CP157672
Coordinates   590838..592271 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain ST-131     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 585838..597271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL174_RS02865 (ABL174_02865) elbB 586530..587183 (+) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -
  ABL174_RS02870 (ABL174_02870) mtgA 587180..587908 (+) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABL174_RS02875 (ABL174_02875) yrbL 587905..588537 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ABL174_RS02880 (ABL174_02880) npr 588750..589022 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABL174_RS02885 (ABL174_02885) rapZ 589019..589873 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ABL174_RS02890 (ABL174_02890) ptsN 589919..590410 (-) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABL174_RS02895 (ABL174_02895) hpf 590528..590815 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABL174_RS02900 (ABL174_02900) rpoN/rpoN1 590838..592271 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ABL174_RS02905 (ABL174_02905) lptB 592319..593044 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABL174_RS02910 (ABL174_02910) lptA 593051..593608 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABL174_RS02915 (ABL174_02915) lptC 593577..594152 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABL174_RS02920 (ABL174_02920) kdsC 594149..594715 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABL174_RS02925 (ABL174_02925) kdsD 594736..595722 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABL174_RS02930 (ABL174_02930) yrbG 595736..596713 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=906215 ABL174_RS02900 WP_000809051.1 590838..592271(-) (rpoN/rpoN1) [Escherichia coli strain ST-131]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=906215 ABL174_RS02900 WP_000809051.1 590838..592271(-) (rpoN/rpoN1) [Escherichia coli strain ST-131]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAAGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGATGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCGATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
AGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGCCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAA
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465