Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB73_RS12635 Genome accession   NZ_CP138940
Coordinates   2231169..2232962 (-) Length   597 a.a.
NCBI ID   WP_413404610.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9509     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2226169..2237962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB73_RS12615 msrB 2226912..2227421 (+) 510 WP_413404618.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB73_RS12620 - 2227351..2228640 (+) 1290 WP_413404616.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB73_RS12625 - 2228654..2230078 (-) 1425 WP_413404614.1 type II secretion system F family protein -
  SIB73_RS12630 pilT 2230082..2231158 (-) 1077 WP_413404612.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB73_RS12635 pilB/pilB1 2231169..2232962 (-) 1794 WP_413404610.1 GspE/PulE family protein Machinery gene
  SIB73_RS12640 grpE 2232992..2233729 (+) 738 WP_413406182.1 nucleotide exchange factor GrpE -
  SIB73_RS12645 dnaJ 2233772..2234902 (+) 1131 WP_413404608.1 molecular chaperone DnaJ -
  SIB73_RS12650 - 2234905..2235147 (+) 243 WP_413404605.1 sulfurtransferase TusA family protein -
  SIB73_RS12655 rsgA 2235167..2236048 (+) 882 WP_413404603.1 ribosome small subunit-dependent GTPase A -
  SIB73_RS12660 - 2236023..2236364 (-) 342 WP_413404601.1 YbaB/EbfC family nucleoid-associated protein -
  SIB73_RS12665 murB 2236389..2237321 (-) 933 WP_413404599.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 65246.39 Da        Isoelectric Point: 4.9713

>NTDB_id=906068 SIB73_RS12635 WP_413404610.1 2231169..2232962(-) (pilB/pilB1) [Synechococcus sp. MIT S9509]
MLIGEAVLPQAGEARTFLRIEAVSDDRRHLELELLLQRAIPGDFGIADQPVLLEELATMGFDPARHAALAESLRKLLDNN
LPLSDEALPLKPPITETPPRHIEVLPGPMTSVSNAAEAETVAETATSYLRDFNVNGVLEADPDEKDADSSKPADLESGLN
DAERSPVIDLVDRILMQALELGASDIHVEPQQAGLQLRFRQDGVLQSQIEPLPSRMVPAVTSRFKIMAELDIAERRLPQD
GRIRRRFQNRTVDFRVNSLPSRYGEKIVLRLLDSSATQLGLDKLISNADALNLVRTLGSKPFGMILVTGPTGSGKSTTLY
SLLAERNEPGINISTIEDPIEYTLPGITQCQVNREKGFDFSQALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVL
TTLHCNDAPSAIARLDEMGVEPFMVSASLIGIVSQRLLRRVCSTCRIAYRPDPQELGRFGLMASNEANVSFFRANHHEGQ
ANPCPHCQGSGYKGRVGVYEVLRMNEALAAAVAKGATTDMVRQLALEGGMKTLLGYSLDLVREGHTTLEEVGRMVLTDAG
LESERRARALSTLTCHGCGGGLQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=906068 SIB73_RS12635 WP_413404610.1 2231169..2232962(-) (pilB/pilB1) [Synechococcus sp. MIT S9509]
ATGTTGATAGGTGAGGCAGTCCTCCCACAAGCGGGGGAAGCCCGCACCTTTCTGCGAATTGAAGCCGTGAGCGACGACCG
TCGGCATCTGGAACTGGAACTTCTGCTGCAGCGAGCCATTCCAGGCGATTTCGGCATCGCTGATCAACCTGTTCTGCTCG
AGGAACTGGCGACGATGGGATTTGATCCTGCTCGTCACGCTGCGCTCGCCGAAAGCCTGAGAAAGCTGCTCGACAACAAC
CTCCCGTTGAGCGATGAAGCACTCCCCCTCAAACCACCCATCACGGAAACACCGCCAAGACATATCGAGGTCTTGCCGGG
ACCCATGACCTCAGTTTCAAACGCTGCAGAGGCCGAAACCGTTGCCGAGACGGCTACCTCCTATCTGAGGGATTTCAACG
TCAATGGGGTGCTGGAAGCTGATCCCGACGAGAAGGATGCCGACTCCTCAAAACCCGCGGATCTTGAATCGGGGCTCAAC
GATGCGGAACGCTCACCGGTGATCGATCTGGTGGATCGCATCCTGATGCAGGCCCTCGAACTGGGTGCCAGCGATATCCA
TGTGGAGCCTCAACAGGCCGGACTCCAGCTGCGCTTTCGTCAGGACGGGGTGCTGCAGAGCCAGATCGAACCTCTGCCCA
GTCGGATGGTGCCTGCGGTGACCTCCAGATTCAAGATCATGGCGGAACTGGACATCGCCGAACGCCGGCTTCCCCAGGAC
GGCCGCATTCGCCGCCGCTTCCAGAACAGGACAGTGGATTTCAGGGTCAACAGTCTGCCGAGCCGCTATGGGGAAAAAAT
CGTTCTGCGGCTTCTGGACAGCTCGGCCACCCAGCTGGGTCTCGACAAACTCATTTCCAATGCTGACGCCCTGAATCTGG
TGCGCACCTTGGGATCCAAGCCCTTCGGAATGATCCTGGTCACAGGACCGACTGGATCCGGTAAGTCGACAACGCTGTAT
TCCCTGCTGGCGGAGCGCAACGAACCAGGAATCAACATCTCCACCATTGAGGACCCCATCGAATACACGCTGCCAGGCAT
CACCCAGTGTCAGGTGAATAGGGAGAAGGGATTTGACTTCAGCCAGGCACTGAGGGCCTTCATGCGCCAGGACCCGGACG
TGCTGCTGGTGGGCGAAACGCGCGATCTGGAAACCGCGAAAACAGCCATCGAAGCCGCACTCACCGGTCACCTGGTGCTG
ACCACCCTTCACTGCAACGATGCTCCCAGCGCCATCGCCCGTCTCGATGAGATGGGTGTGGAGCCTTTCATGGTCAGCGC
CTCACTGATCGGGATCGTCTCCCAGCGTCTGCTCCGACGGGTCTGCTCGACTTGCAGGATCGCCTACCGGCCTGATCCCC
AAGAACTGGGTCGTTTCGGCCTGATGGCGAGCAATGAAGCGAACGTGAGCTTTTTCAGAGCCAACCACCATGAAGGGCAG
GCCAACCCATGCCCCCATTGCCAGGGCAGTGGTTACAAGGGACGCGTGGGGGTCTACGAAGTGCTGCGCATGAATGAAGC
TCTCGCGGCCGCCGTAGCCAAGGGCGCCACCACAGACATGGTGCGACAGCTCGCTCTGGAGGGAGGGATGAAAACCTTGC
TGGGTTACAGCCTTGACCTGGTGAGGGAGGGACACACGACCCTGGAGGAAGTGGGCAGGATGGTTCTCACAGACGCCGGG
CTGGAATCGGAACGACGCGCACGCGCTCTCAGCACCCTCACCTGCCATGGCTGTGGTGGCGGACTCCAGGAAGGTTGGCT
GGAATGTCCCTACTGCCTGACACCACGTCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(174-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

61.46

82.58

0.508