Detailed information NTG0090596

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: BSG8_RS19245
• Genome accession: NZ_AP025224
• Coordinates: 3574966..3576111 (+)
• Length: 381 a.a.
• NCBI ID: WP_014481226.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. natto strain G8
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3569966..3581111

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSG8_RS19225 (BSG8_38110)	albF	3569982..3571262 (+)	1281	WP_014481221.1	pitrilysin family protein	-
BSG8_RS19230 (BSG8_38120)	albG	3571259..3571960 (+)	702	WP_014481222.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
BSG8_RS19235 (BSG8_38130)	ywhL	3571966..3573342 (-)	1377	WP_014481223.1	YncE family protein	-
BSG8_RS19240 (BSG8_38140)	ywhK	3573381..3574736 (-)	1356	WP_014481224.1	YncE family protein	-
BSG8_RS21805	-	3574733..3574963 (-)	231	Protein_3794	hypothetical protein	-
BSG8_RS19245 (BSG8_38150)	rapF	3574966..3576111 (+)	1146	WP_014481226.1	response regulator aspartate phosphatase RapF	Regulator
BSG8_RS19250 (BSG8_38160)	phrF	3576095..3576214 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
BSG8_RS19255 (BSG8_38170)	-	3576312..3576739 (+)	428	Protein_3797	YbaK/EbsC family protein	-
BSG8_RS19260 (BSG8_38180)	speB	3576804..3577676 (-)	873	WP_003227545.1	agmatinase	-
BSG8_RS19265 (BSG8_38190)	speE	3577737..3578567 (-)	831	WP_003227543.1	spermidine synthase	-
BSG8_RS19270 (BSG8_38200)	pbpG	3578769..3580844 (+)	2076	WP_014481227.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45627.12 Da
• Isoelectric Point: 5.1503

>NTDB_id=90596 BSG8_RS19245 WP_014481226.1 3574966..3576111(+) (rapF) [Bacillus subtilis subsp. natto strain G8]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=90596 BSG8_RS19245 WP_014481226.1 3574966..3576111(+) (rapF) [Bacillus subtilis subsp. natto strain G8]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	99.738	100	0.997
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572