Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ86_RS01935 Genome accession   NZ_AP027800
Coordinates   412713..413390 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 2017.10.01CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407713..418390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ86_RS01920 (VEE56_03810) chiA 409080..411773 (+) 2694 WP_094322432.1 bifunctional chitinase/lysozyme -
  AABJ86_RS01925 (VEE56_03820) bfd 411942..412136 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABJ86_RS01930 (VEE56_03830) bfr 412208..412684 (+) 477 WP_000675504.1 bacterioferritin -
  AABJ86_RS01935 (VEE56_03840) pilD 412713..413390 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  AABJ86_RS01940 (VEE56_03850) gspM 413390..413851 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AABJ86_RS01945 (VEE56_03860) gspL 413848..415011 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  AABJ86_RS01950 (VEE56_03870) gspK 415026..416009 (-) 984 Protein_387 type II secretion system minor pseudopilin GspK -
  AABJ86_RS01955 (VEE56_03880) gspJ 416002..416589 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABJ86_RS01960 (VEE56_03890) gspI 416582..416959 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABJ86_RS01965 (VEE56_03900) gspH 416956..417465 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  AABJ86_RS01970 (VEE56_03910) gspG 417473..417910 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=90438 AABJ86_RS01935 WP_000178161.1 412713..413390(-) (pilD) [Escherichia coli strain 2017.10.01CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=90438 AABJ86_RS01935 WP_000178161.1 412713..413390(-) (pilD) [Escherichia coli strain 2017.10.01CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment