Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Cthiooxydans_RS24365 Genome accession   NZ_AP025193
Coordinates   5279026..5280627 (-) Length   533 a.a.
NCBI ID   WP_087864602.1    Uniprot ID   -
Organism   Comamonas thiooxydans strain NR4028     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5274026..5285627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cthiooxydans_RS24340 (Cthiooxydans_47520) - 5274113..5274604 (-) 492 WP_003068209.1 PIN domain-containing protein -
  Cthiooxydans_RS24345 (Cthiooxydans_47530) - 5274601..5275899 (-) 1299 WP_054073672.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  Cthiooxydans_RS24350 (Cthiooxydans_47540) - 5276079..5276417 (+) 339 WP_039050220.1 tRNA-binding protein -
  Cthiooxydans_RS24355 (Cthiooxydans_47550) - 5276475..5277707 (+) 1233 WP_087864603.1 DUF711 family protein -
  Cthiooxydans_RS24360 (Cthiooxydans_47560) - 5277843..5278766 (+) 924 WP_003068201.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  Cthiooxydans_RS24365 (Cthiooxydans_47570) rpoN/rpoN1 5279026..5280627 (-) 1602 WP_087864602.1 RNA polymerase factor sigma-54 Machinery gene
  Cthiooxydans_RS24370 (Cthiooxydans_47580) lptB 5280773..5281555 (-) 783 WP_003068196.1 LPS export ABC transporter ATP-binding protein -
  Cthiooxydans_RS24375 (Cthiooxydans_47590) lptA 5281552..5282208 (-) 657 WP_003068194.1 lipopolysaccharide transport periplasmic protein LptA -
  Cthiooxydans_RS24380 (Cthiooxydans_47600) - 5282339..5282989 (-) 651 WP_012840180.1 thiol:disulfide interchange protein DsbA/DsbL -
  Cthiooxydans_RS24385 (Cthiooxydans_47610) - 5283150..5283854 (-) 705 WP_087864601.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59144.35 Da        Isoelectric Point: 4.6361

>NTDB_id=90412 Cthiooxydans_RS24365 WP_087864602.1 5279026..5280627(-) (rpoN/rpoN1) [Comamonas thiooxydans strain NR4028]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAETAGDAPVDVAASASEYSTSDGIHAE
RDASQAQQATETVAETSNPETPETLNWEAEGLDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADTAALRFLIESLSDDGYLEDSLQELAQSLADDDLEEREELEQHFAIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMSRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVIPDVIVRPVRAGSGRVDFSVQLNPEVMPRLRVHDIYASVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDARKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=90412 Cthiooxydans_RS24365 WP_087864602.1 5279026..5280627(-) (rpoN/rpoN1) [Comamonas thiooxydans strain NR4028]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTATCGCTTACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTCTCCACCCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAACGTGCGGGCGATGAGG
CAGAAACTGCGGGGGATGCTCCTGTAGATGTAGCTGCTAGCGCCAGTGAATACAGCACTTCAGATGGTATTCATGCTGAA
AGAGATGCATCGCAAGCGCAACAAGCTACTGAAACGGTAGCGGAGACATCAAACCCCGAAACCCCGGAAACGCTGAACTG
GGAGGCCGAGGGCCTGGACGGCCAGGACGGGGAATGGGGCGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGACGGCGATGACGAAACCGATGCCATCGATCTGGCGCGTGAGCACCAGAGCCTGACCGACTTT
CTCCATCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCGGACACGGCGGCACTGCGCTTTCTCATCGAGTCGCTCAGCGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGTTGGCGCAGAGCCTGGCCGACGACGACCTGGAAGAGCGCGAGGAACTGG
AGCAGCACTTTGCCATTGCGCTGAAGCTGCTGCAAAGCCTGGAGCCCGTGGGCGTGGGTGCGCGCGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGATCTGATCCGCACGGCCGAGGACGAGGGCACGACCGAGGCGCATATGAGCCGGCTGCTGCT
GGCGCAGGCCATCTGTGCCCAGCCCATGGACCTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCGC
GCGAGGAACAGCTGCGCGAGGCCATGGCCCTGATCGCCAGGCTGGAGCCGCGTCCGGGGCGCCGCTTTGTCGACGTGGAG
CGCCATATCGTGATCCCCGATGTCATCGTGCGCCCGGTGCGGGCCGGCTCGGGCCGGGTGGACTTTTCCGTGCAGCTCAA
CCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCAGCGTGCTGCGCCGCCACAAGAACAGCGACGGCCATG
CGGCGCTGCAGCAGCAACTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTTGACACCATCTTGCGTGTT
TCCGAAGCCATCGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGATAT
CGCCGACACGCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACTACGGCCAAGTACATGGCCACGCCCCAGGGTACCT
TCGAGCTCAAGTATTTCTTCGGTTCGGGCCTGGGCACGGAAACCGGCGGCAGTGCTTCCAGCACGGCCGTGCGTGCGCTG
ATCAAGCAGTTTGTCGATGCCGAAGATGCCAGAAAGCCGCTGTCGGACGCCAAGCTGGCCGATATGCTCAAGGAGCAGGG
CATCGAGTGCGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.119

100

0.514

Multiple sequence alignment