Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ABKA22_RS03230 Genome accession   NZ_CP157287
Coordinates   605031..607259 (+) Length   742 a.a.
NCBI ID   WP_348619136.1    Uniprot ID   -
Organism   Lactococcus lactis strain BIM B-1834     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 600031..612259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKA22_RS03195 (ABKA22_03195) - 600519..601187 (+) 669 WP_003129542.1 hypothetical protein -
  ABKA22_RS03200 (ABKA22_03200) - 601357..602253 (+) 897 WP_003129543.1 SPFH domain-containing protein -
  ABKA22_RS03210 (ABKA22_03210) - 602628..602888 (+) 261 WP_003129544.1 SemiSWEET family transporter -
  ABKA22_RS03220 (ABKA22_03220) rnz 603246..604169 (+) 924 WP_348619134.1 ribonuclease Z -
  ABKA22_RS03225 (ABKA22_03225) - 604162..604864 (+) 703 Protein_603 SDR family oxidoreductase -
  ABKA22_RS03230 (ABKA22_03230) recJ 605031..607259 (+) 2229 WP_348619136.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ABKA22_RS03235 (ABKA22_03235) - 607382..607894 (+) 513 WP_012897343.1 adenine phosphoribosyltransferase -
  ABKA22_RS03240 (ABKA22_03240) rpoE 608110..608673 (+) 564 WP_003129551.1 DNA-directed RNA polymerase subunit delta -
  ABKA22_RS03245 (ABKA22_03245) mltG 608810..610462 (+) 1653 WP_014570395.1 endolytic transglycosylase MltG -
  ABKA22_RS03250 (ABKA22_03250) greA 610522..610995 (+) 474 WP_025016857.1 transcription elongation factor GreA -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83540.66 Da        Isoelectric Point: 4.7534

>NTDB_id=904004 ABKA22_RS03230 WP_348619136.1 605031..607259(+) (recJ) [Lactococcus lactis strain BIM B-1834]
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHDPFLLHDMEKATGRILSAIEQ
GENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELIKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKMIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWNGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEETPGDLAGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=904004 ABKA22_RS03230 WP_348619136.1 605031..607259(+) (recJ) [Lactococcus lactis strain BIM B-1834]
ATGATAAAAGCAAAATATGATTGGAAAGTGGCTGATACAGCCATTTCCGAAGATTTTCTAAAGATAGCGAAAAAACATAA
ATTAGATGAATTGACAAGTCGCGTGCTTTATCAAAGAGGAATTCATGCAGAAGCTGAAATTGAGCAATTTTTAAAGCCAA
GTTTAGAAAATTTACATGACCCTTTTCTACTTCATGATATGGAAAAAGCAACAGGGCGTATTTTATCAGCAATTGAGCAA
GGTGAAAACATCTTAATTTATGGTGACTATGATGCTGACGGAATGACTGCATCATCAGTCATGAAATCCGCTCTGGATGA
ATTAGGAGCTGAGGCTCAAGTTTATTTACCCAATCGTTTTACTGACGGTTACGGGCCAAATCTTGACGTTTATCAGTACT
ATATTAAGAATGAAAATATCAATCTTATTATTACTGTAGATAATGGGGTTGCTGGCTTGGAGGCAATTACCTGGGCACAG
GAAAATGGAGTGGATGTAATTGTTACCGACCATCACTCAATTCCTGACCAACTTCCCCCTGCTTATGCCATTGTCCACCC
CGAACATCCTGACAGTCAATATCCTTTCAAATATTTGGCAGGAGTTGGTGTCGCCTTCAAAGTTGCCTGTGCTCTTTTAG
AATATGCACCCAGTGAAATGCTTGATTTAGTTGCAATTGGTACAATTGCTGACATGGTCAGTTTGACTGATGAAAACCGA
ATTTTAGTGGCTCATGGTCTAAAAGTTTTGGCCCAAACAGAGCGTGCCGGCCTACAAGAATTAATAAAGTATGCCGGGGT
TGATTTTGATAAAATCACTGAGGAAACGGTTGGTTTTCAAATTGCTCCCCGTTTAAACGCTTTGGGACGTCTCGATGACC
CAAACCCAGCCATTGAATTGCTGACAGGTTGGGATGAAGATGAAGCGCATGAAATTGCCAAAATGATTGACCAAAAAAAT
TCAGAGCGAAAAGAAATTGTGGAAAAAATTCACAATGAAGCTCTGAGTATGCTGACAGATGAACCTGTTCAGATTTTATA
TCATAAGGACTGGCATAAAGGTGTCTTAGGAATTGTTGCGGGTCGCTTGCTAGAAGCCATACATAAGCCAGTAATCATGC
TGGCCCAAGAAGATGGGATTTTGCGTGGGTCTGCTCGGTCAATCGAAAATTTTGATATTTTCAAGGCATTAAATGCCCAT
CGTGAACTTTTTATTGCTTTTGGTGGTCATAAACAAGCCGCAGGGATGACTTTAAGTCTGGAAAACGTAGAAGCAGTTAA
AAAGGCGATGATTGATTATATCGTGGACAATCATTTAGATATGTCTAAAAAAAGTCCCTTAGAAATTGCTGACCGTTGCC
ACTTAGACGATATTTCGCTCTCAACAATCACTAATTTAGCAAAATTGGCTCCCTTTGGCATGGATAATCCTAAGCCTCGA
TTTTTAATCGAAGATTATAAGATGATTCAAAGCCGAAGTATGGGGAAAGACAATGCTCATCTTAAATTAAAAATTCAGGA
AGAAAAGCAACAAATTGATGCCGTTTATTTCCAACATGGTTCAGAAGAATTGGAATTCGAACAGGCACAAACAAAATTAG
TCGCAACACTTTCAAGTAATAGTTGGAATGGAAATACGAGTCTTCAATTAATGATTGAAGATGCTGATTCAGTTGGTGTC
GAACTTCTTGATATAAGAAGTAAACAAATTCCTATCCCAAAAGAGGCTAATATTTTTTCTCAAAATCAGCTAAAACATGG
TATAATGGAAGATGTGCTTGTCATCGAAGAGACACCTGGAGATTTAGCAGGTTTGTCAGTATTAAAAGAAGCTGTCAGTA
AAGCAGCGTCGGATGATTTTAAAATGATTTATTTTAAAAATAAAATCACTGATAGCTATTATTTAACAGGAAGTGGAACA
CGAGAAGAATTTGCAAGACTTTATAAAGCAATTTATCAATTTCCAGAATTTGATATTCGCTATAAATTAAAATCACTTGC
GGATTATTTGAAAATCCCTCATCTCTTGCTTGTCAAGATGATAAAAATCTTTGAGGAACTTGAATTTGTCAGCATTGACA
ATGGCTTAATGACCGTTAATAAGACGGCCGATAAACGCGAAATTTCTGAGAGTACAATTTATCAAGAACTAGAAAAAATT
GTAAAAATGCAAGAACTTTTTGCTTTAGCACCCGTCAAAGAAATTTATCAAAATTTATTAGAAAAATAA

Domains

Predicted by InterProScan.

DHH family (DHH)

(83-228)

RecJ OB domain (RecJ_OB)

(454-554)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(592-737)

DHHA1 domain (DHHA1)

(317-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

56.779

100

0.57
  recJ Bacillus subtilis subsp. subtilis str. 168

36.714

100

0.385