Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SD459_RS12360 Genome accession   NZ_CP138627
Coordinates   2496623..2497693 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain AYGS-17     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491623..2502693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD459_RS12310 (SD459_12305) sipW 2491682..2492266 (-) 585 WP_014418370.1 signal peptidase I SipW -
  SD459_RS12315 (SD459_12310) tapA 2492238..2492909 (-) 672 WP_025649852.1 amyloid fiber anchoring/assembly protein TapA -
  SD459_RS12320 (SD459_12315) - 2493168..2493497 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  SD459_RS12325 (SD459_12320) - 2493538..2493717 (-) 180 WP_003153093.1 YqzE family protein -
  SD459_RS12330 (SD459_12325) comGG 2493774..2494151 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  SD459_RS12335 (SD459_12330) comGF 2494152..2494547 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  SD459_RS12340 (SD459_12335) comGE 2494561..2494875 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  SD459_RS12345 (SD459_12340) comGD 2494859..2495296 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  SD459_RS12350 (SD459_12345) comGC 2495286..2495552 (-) 267 WP_050496152.1 competence type IV pilus major pilin ComGC Machinery gene
  SD459_RS12355 (SD459_12350) comGB 2495599..2496636 (-) 1038 WP_053284924.1 competence type IV pilus assembly protein ComGB Machinery gene
  SD459_RS12360 (SD459_12355) comGA 2496623..2497693 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  SD459_RS12365 (SD459_12360) - 2497886..2498836 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  SD459_RS12370 (SD459_12365) - 2498982..2500283 (+) 1302 WP_025649848.1 hemolysin family protein -
  SD459_RS12375 (SD459_12370) - 2500321..2501151 (-) 831 WP_025649847.1 STAS domain-containing protein -
  SD459_RS12385 (SD459_12380) - 2501358..2501738 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  SD459_RS12390 (SD459_12385) - 2501975..2502220 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=903751 SD459_RS12360 WP_012117985.1 2496623..2497693(-) (comGA) [Bacillus velezensis strain AYGS-17]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=903751 SD459_RS12360 WP_012117985.1 2496623..2497693(-) (comGA) [Bacillus velezensis strain AYGS-17]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374