Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RZE78_RS02535 Genome accession   NZ_CP138515
Coordinates   552112..553704 (+) Length   530 a.a.
NCBI ID   WP_319429040.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 2u118     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 547112..558704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE78_RS02510 (RZE78_02510) argS 547219..548904 (+) 1686 WP_319429030.1 arginine--tRNA ligase -
  RZE78_RS02515 (RZE78_02515) - 548912..549640 (+) 729 WP_319429032.1 SPOR domain-containing protein -
  RZE78_RS02520 (RZE78_02520) - 549785..550432 (+) 648 WP_319429034.1 thiol:disulfide interchange protein DsbA/DsbL -
  RZE78_RS02525 (RZE78_02525) lptA 550584..551303 (+) 720 WP_319429036.1 lipopolysaccharide transport periplasmic protein LptA -
  RZE78_RS02530 (RZE78_02530) lptB 551300..552094 (+) 795 WP_319429038.1 LPS export ABC transporter ATP-binding protein -
  RZE78_RS02535 (RZE78_02535) rpoN/rpoN1 552112..553704 (+) 1593 WP_319429040.1 RNA polymerase factor sigma-54 Machinery gene
  RZE78_RS02540 (RZE78_02540) - 553715..555319 (+) 1605 WP_319429042.1 phospholipase D family protein -
  RZE78_RS02545 (RZE78_02545) - 555330..555977 (-) 648 WP_319429044.1 LysE family translocator -
  RZE78_RS02550 (RZE78_02550) - 555982..556617 (-) 636 WP_319429046.1 YceI family protein -
  RZE78_RS02555 (RZE78_02555) - 556661..557233 (-) 573 WP_405548912.1 YceI family protein -
  RZE78_RS02560 (RZE78_02560) - 557230..557859 (-) 630 WP_319429048.1 cytochrome b -
  RZE78_RS02565 (RZE78_02565) - 557950..558414 (-) 465 WP_319429049.1 hypothetical protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58482.80 Da        Isoelectric Point: 4.7226

>NTDB_id=903279 RZE78_RS02535 WP_319429040.1 552112..553704(+) (rpoN/rpoN1) [Variovorax paradoxus strain 2u118]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAIDTPVPRDEAGEAAEGE
FSAAPASSTTSTSSETASTAESDVPAADATEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESL
QSFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPAGVGARDL
GECLCIQLRALASESETEEQALVRKTAIAICKQPMELLARRDFKRLATLTRTNEELVRSALQVISRLEPKPGRRFVDVER
NIVIPDVIVTKIGRGTNAKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNA
IVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPFGTVELKYFFGSALGTETGGNASSTAVRALIKQ
FVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=903279 RZE78_RS02535 WP_319429040.1 552112..553704(+) (rpoN/rpoN1) [Variovorax paradoxus strain 2u118]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCACCTGGCGCTCACGCCCCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAACTGAGCCAGGAAGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CCGCGCGCGAGGAATTCGGGCTCGATGCCATCGACACGCCGGTGCCGCGCGACGAGGCCGGTGAAGCCGCCGAGGGCGAA
TTCAGCGCAGCCCCGGCCAGCAGCACGACCAGCACGTCCAGCGAAACCGCCTCCACGGCCGAGTCCGATGTTCCCGCCGC
CGATGCCACCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAATGGGGCAGCG
ATGCCCCCGCGCGCCAGAACAACCTCGGCGACGACGAGCGCGCCGACGCCACCGAACTCGCACGCAGCCAGGAATCGCTG
CAGTCCTTCCTGCACCGGCAGGCGCTGAGCCTGCGGCTCAACGAAAACGACAGCGCCGCGCTGCGCTTCCTGATCGAATC
GCTCAACGACGACGGCTATCTCGAAGACTCCCTGCCGGCGCTGGCCTCGGGCCTTGCGGGCGACGACAACGACCAGTTCG
ACGAGCTGGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCGGCCGGCGTCGGCGCGCGCGACCTG
GGCGAATGCCTTTGCATCCAGCTGCGCGCACTCGCGAGCGAAAGCGAGACCGAGGAACAGGCGCTGGTGCGCAAGACCGC
CATCGCCATCTGCAAGCAGCCGATGGAGCTACTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGCACCAACG
AAGAGCTGGTGCGCTCGGCGCTGCAGGTCATCTCGCGCCTGGAGCCCAAGCCGGGCCGCCGCTTCGTCGACGTCGAGCGC
AACATCGTCATTCCCGACGTGATCGTCACCAAGATCGGCCGCGGCACCAATGCCAAGTTCCGCGTCATGCTCAACCCCGA
GGTGATGCCGCGGCTGCGCGTGCACGACATCTATGCCGGCGCGCTCAAGTCGCACAAGGGCGAAGGCAGCCAGGCGCTGT
CGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCC
ATCGTCGAGCGGCAGAAGAGCTTCTTCGTGCACGGCGAGCTCGCGATGCGGCCCCTGGTGCTGCGCGAGATTGCCGACGA
ACTGGGCCTGCACGAATCGACCATCTCGCGCGTGACCACGGCCAAGTACATGTCCACGCCTTTCGGCACGGTCGAGCTCA
AGTACTTCTTCGGCTCGGCGCTCGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCGGTGCGCGCGCTGATCAAGCAG
TTCGTGAGCTCCGAGAGCATCAAGAAGCCGCTGTCCGACAGCCAGATCTCCGAGATGCTCAAGGAGCAGGGCATCGAGTG
CGCGCGCCGCACCGTGGCCAAGTACCGCGAGGCCCTGCGCATCGCACCGGCCAACCTTCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-358)

Sigma-54, DNA binding domain (Sigma54_DBD)

(372-528)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.977

100

0.521