Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RZE79_RS02545 Genome accession   NZ_CP138496
Coordinates   560415..562007 (+) Length   530 a.a.
NCBI ID   WP_153280971.1    Uniprot ID   -
Organism   Variovorax sp. EBFNA2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 555415..567007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE79_RS02525 (RZE79_02525) - 557069..557815 (+) 747 WP_153280974.1 SPOR domain-containing protein -
  RZE79_RS02530 (RZE79_02530) - 557978..558622 (+) 645 WP_192194669.1 thiol:disulfide interchange protein DsbA/DsbL -
  RZE79_RS02535 (RZE79_02535) lptA 558744..559466 (+) 723 WP_319358893.1 lipopolysaccharide transport periplasmic protein LptA -
  RZE79_RS02540 (RZE79_02540) lptB 559463..560260 (+) 798 WP_101488358.1 LPS export ABC transporter ATP-binding protein -
  RZE79_RS02545 (RZE79_02545) rpoN/rpoN1 560415..562007 (+) 1593 WP_153280971.1 RNA polymerase factor sigma-54 Machinery gene
  RZE79_RS02550 (RZE79_02550) - 562018..563613 (+) 1596 WP_319358894.1 phospholipase D family protein -
  RZE79_RS02555 (RZE79_02555) - 563625..564269 (-) 645 WP_153280969.1 LysE family translocator -
  RZE79_RS02560 (RZE79_02560) - 564292..564927 (-) 636 WP_153280968.1 YceI family protein -
  RZE79_RS02565 (RZE79_02565) - 564966..565547 (-) 582 WP_153280967.1 YceI family protein -
  RZE79_RS02570 (RZE79_02570) - 565544..566155 (-) 612 WP_319358895.1 cytochrome b -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58462.76 Da        Isoelectric Point: 4.6871

>NTDB_id=903155 RZE79_RS02545 WP_153280971.1 560415..562007(+) (rpoN/rpoN1) [Variovorax sp. EBFNA2]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAVDTPVPRDDGGDTGEGD
FAPSAPSASTASSEATTSTPESDGPAAEITEREPDWEGDGTVEMAPDDSEWGSDAPARQNNLGDDERADATELARSQESL
QSFLHRQALTLRLNENDCAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPAGVGARDL
GECLSIQLKALANEDETEAEALVRKTAIAICKQPMELLARRDFKRLATLTRTNEELVRSALQIIARLEPKPGRRFVDVER
NIVVPDVIVTKTGRGTNIKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNA
IVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPFGTVELKYFFGSALGTETGGNASSTAVRALIKQ
FVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=903155 RZE79_RS02545 WP_153280971.1 560415..562007(+) (rpoN/rpoN1) [Variovorax sp. EBFNA2]
ATGAAGCAAGGGCTGTCCCTTCGCGTCTCGCAGCATCTGGCGCTGACGCCTCAGCTGCAGCAGTCCATTCGCCTGCTCCA
ACTCTCCACGCTCGAACTGAGCCAGGAGGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CCGCGCGCGAGGAGTTCGGGCTCGATGCTGTCGACACGCCCGTGCCGCGCGACGACGGCGGTGACACCGGCGAGGGCGAT
TTCGCGCCGTCCGCCCCCAGTGCCTCCACCGCTTCCAGCGAAGCGACCACGTCCACACCGGAATCCGACGGCCCCGCGGC
CGAGATCACCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGAGATGGCGCCCGACGACAGCGAGTGGGGCAGCG
ATGCGCCCGCGCGCCAGAACAACCTCGGCGACGACGAGCGTGCCGACGCCACCGAACTCGCGCGCAGCCAGGAGTCGCTG
CAATCGTTCCTGCACCGCCAGGCGCTGACCCTGCGGCTCAACGAAAACGACTGCGCCGCGCTGCGCTTTTTGATCGAATC
GCTCAACGACGACGGTTACCTCGAAGACTCGCTGCCCGCGCTGGCCTCGGGCCTGGCGGGCGATGACAACGACCAGTTCG
ACGACCTCGTGCACCACTTCCAGGTGGCGCTGGGCCTGCTGCAGAGCCTGGAGCCCGCCGGTGTCGGCGCGCGCGACCTC
GGCGAATGCCTGAGCATTCAGCTGAAGGCGCTCGCGAACGAAGACGAGACCGAGGCCGAAGCGCTTGTGCGCAAGACCGC
CATCGCGATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGGACGAATG
AAGAGCTGGTGCGCTCGGCGCTGCAGATCATTGCGCGGCTCGAGCCCAAGCCGGGGCGCCGTTTTGTCGACGTCGAACGC
AACATCGTCGTTCCCGACGTGATCGTCACCAAGACGGGCCGCGGCACCAACATCAAGTTCCGCGTCATGCTGAACCCCGA
GGTCATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGTGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGT
CGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCC
ATCGTCGAACGGCAGAAGAGCTACTTCGTGCACGGCGAACTCGCGATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGA
ACTGGGCCTGCACGAATCGACGATTTCGCGCGTGACCACGGCCAAGTACATGTCGACGCCGTTCGGCACGGTCGAGCTCA
AGTACTTCTTCGGGTCGGCGCTGGGCACCGAGACCGGCGGCAATGCATCGAGCACCGCGGTGCGCGCGCTCATCAAGCAG
TTCGTGAGCTCCGAGAGCATCAAGAAGCCGCTGTCGGACAGCCAGATTTCCGAGATGCTGAAAGAGCAGGGCATCGAGTG
CGCGCGCCGCACCGTGGCCAAGTACCGCGAAGCGCTGCGCATCGCGCCCGCCAACCTGCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-358)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(372-528)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.844

100

0.511