Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SGJ46_RS11915 Genome accession   NZ_CP138461
Coordinates   2463440..2464237 (-) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain CZH-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2458440..2469237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGJ46_RS11895 - 2459483..2460187 (-) 705 WP_005182401.1 YfgM family protein -
  SGJ46_RS11900 hisS 2460218..2461513 (-) 1296 WP_177155339.1 histidine--tRNA ligase -
  SGJ46_RS11905 ispG 2461517..2462638 (-) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SGJ46_RS11910 - 2462661..2463449 (-) 789 WP_005182393.1 helix-turn-helix domain-containing protein -
  SGJ46_RS11915 pilF 2463440..2464237 (-) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SGJ46_RS11920 rlmN 2464273..2465508 (-) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SGJ46_RS11925 ndk 2465631..2466062 (-) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  SGJ46_RS11930 iscX 2466172..2466372 (-) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  SGJ46_RS11935 - 2466467..2467018 (-) 552 WP_005182381.1 phosphatase PAP2 family protein -
  SGJ46_RS11940 - 2467051..2468334 (-) 1284 WP_329414984.1 glycosyltransferase family 1 protein -
  SGJ46_RS11945 - 2468502..2468921 (-) 420 WP_005182377.1 hypothetical protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=902933 SGJ46_RS11915 WP_104470591.1 2463440..2464237(-) (pilF) [Acinetobacter indicus strain CZH-5]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=902933 SGJ46_RS11915 WP_104470591.1 2463440..2464237(-) (pilF) [Acinetobacter indicus strain CZH-5]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCACTGGAAACAGATTCGCGTGATTCTATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGTAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTCAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCTCTGCAGGCGAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCAGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTGGGACAAAAAAATCAAGGTG
CGCGTGCATTGTGGATTGGGATCCGTGTTGCACGAGCAAATGGCGATCAGTTGGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732