Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   LPC25_RS00205 Genome accession   NZ_AP025176
Coordinates   47765..49114 (-) Length   449 a.a.
NCBI ID   WP_000815652.1    Uniprot ID   Q8VUV4
Organism   Staphylococcus aureus strain TPS5614     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39350..54776 47765..49114 within 0


Gene organization within MGE regions


Location: 39350..54776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPC25_RS00165 (TPS5614TP_00330) mecA 39350..41359 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  LPC25_RS00170 (TPS5614TP_00340) - 41456..42442 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  LPC25_RS00175 (TPS5614TP_00350) - 42432..42674 (+) 243 Protein_32 type I restriction enzyme endonuclease domain-containing protein -
  LPC25_RS00180 (TPS5614TP_00360) - 42665..44275 (-) 1611 Protein_33 IS1182 family transposase -
  LPC25_RS00185 (TPS5614TP_00370) - 44324..44830 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  LPC25_RS00190 (TPS5614TP_00380) - 44845..45156 (-) 312 WP_000700851.1 DUF960 family protein -
  LPC25_RS00195 (TPS5614TP_00390) - 45243..45593 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LPC25_RS00200 (TPS5614TP_00400) ccrB 46115..47743 (-) 1629 WP_001186604.1 cassette chromosome recombinase CcrB Machinery gene
  LPC25_RS00205 (TPS5614TP_00410) ccrA 47765..49114 (-) 1350 WP_000815652.1 cassette chromosome recombinase CcrA Machinery gene
  LPC25_RS00210 (TPS5614TP_00420) cch1 49348..51135 (-) 1788 WP_000122621.1 cassette chromosome replicative helicase -
  LPC25_RS00215 (TPS5614TP_00430) ssb 51135..51425 (-) 291 WP_001036437.1 cassette chromosome ssDNA-binding protein -
  LPC25_RS00220 (TPS5614TP_00440) - 51564..52613 (+) 1050 WP_000613248.1 hypothetical protein -
  LPC25_RS00225 (TPS5614TP_00450) - 53286..54776 (+) 1491 WP_000554504.1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52616.30 Da        Isoelectric Point: 10.0062

>NTDB_id=90261 LPC25_RS00205 WP_000815652.1 47765..49114(-) (ccrA) [Staphylococcus aureus strain TPS5614]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHILTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQAMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=90261 LPC25_RS00205 WP_000815652.1 47765..49114(-) (ccrA) [Staphylococcus aureus strain TPS5614]
ATGAAACAAGTTATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGTGTTCATATCTTAAGTGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGATTATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGTCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACATATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATAAATGGGCATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAGCCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VUV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

74.833

100

0.748

Multiple sequence alignment