Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABH545_RS27525 Genome accession   NZ_CP157151
Coordinates   5127856..5129289 (+) Length   477 a.a.
NCBI ID   WP_000809047.1    Uniprot ID   -
Organism   Escherichia coli strain UTAK-6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5122856..5134289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABH545_RS27495 (ABH545_27495) yrbG 5123414..5124391 (+) 978 WP_000922872.1 calcium/sodium antiporter -
  ABH545_RS27500 (ABH545_27500) kdsD 5124405..5125391 (+) 987 WP_001368728.1 arabinose-5-phosphate isomerase KdsD -
  ABH545_RS27505 (ABH545_27505) kdsC 5125412..5125978 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABH545_RS27510 (ABH545_27510) lptC 5125975..5126550 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABH545_RS27515 (ABH545_27515) lptA 5126519..5127076 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABH545_RS27520 (ABH545_27520) lptB 5127083..5127808 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABH545_RS27525 (ABH545_27525) rpoN/rpoN1 5127856..5129289 (+) 1434 WP_000809047.1 RNA polymerase factor sigma-54 Machinery gene
  ABH545_RS27530 (ABH545_27530) hpf 5129312..5129599 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABH545_RS27535 (ABH545_27535) ptsN 5129717..5130208 (+) 492 WP_001299132.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABH545_RS27540 (ABH545_27540) rapZ 5130254..5131108 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ABH545_RS27545 (ABH545_27545) npr 5131105..5131377 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABH545_RS27550 (ABH545_27550) yrbL 5131591..5132223 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ABH545_RS27555 (ABH545_27555) mtgA 5132220..5132948 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABH545_RS27560 (ABH545_27560) elbB 5132945..5133598 (-) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54051.83 Da        Isoelectric Point: 4.3447

>NTDB_id=902401 ABH545_RS27525 WP_000809047.1 5127856..5129289(+) (rpoN/rpoN1) [Escherichia coli strain UTAK-6]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=902401 ABH545_RS27525 WP_000809047.1 5127856..5129289(+) (rpoN/rpoN1) [Escherichia coli strain UTAK-6]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGATGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465