Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABK30_RS01920 Genome accession   NZ_AP027783
Coordinates   418984..419661 (-) Length   225 a.a.
NCBI ID   WP_000178155.1    Uniprot ID   -
Organism   Escherichia coli strain 2017-03-3CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413984..424661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABK30_RS01905 (VEE74_03770) chiA 415351..418044 (+) 2694 WP_024250449.1 bifunctional chitinase/lysozyme -
  AABK30_RS01910 (VEE74_03780) bfd 418213..418407 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABK30_RS01915 (VEE74_03790) bfr 418479..418955 (+) 477 WP_000675504.1 bacterioferritin -
  AABK30_RS01920 (VEE74_03800) pilD 418984..419661 (-) 678 WP_000178155.1 prepilin peptidase GspO Machinery gene
  AABK30_RS01925 (VEE74_03810) gspM 419661..420122 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AABK30_RS01930 (VEE74_03820) gspL 420119..421281 (-) 1163 Protein_383 type II secretion system protein GspL -
  AABK30_RS01935 (VEE74_03830) gspK 421296..422279 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  AABK30_RS01940 (VEE74_03840) gspJ 422272..422859 (-) 588 WP_000610636.1 type II secretion system minor pseudopilin GspJ -
  AABK30_RS01945 (VEE74_03850) gspI 422852..423229 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABK30_RS01950 (VEE74_03860) gspH 423226..423735 (-) 510 WP_134778497.1 type II secretion system minor pseudopilin GspH -
  AABK30_RS01955 (VEE74_03870) gspG 423743..424180 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24879.74 Da        Isoelectric Point: 7.6578

>NTDB_id=90202 AABK30_RS01920 WP_000178155.1 418984..419661(-) (pilD) [Escherichia coli strain 2017-03-3CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=90202 AABK30_RS01920 WP_000178155.1 418984..419661(-) (pilD) [Escherichia coli strain 2017-03-3CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCACCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment