Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABH544_RS02610 Genome accession   NZ_CP157093
Coordinates   540814..542247 (-) Length   477 a.a.
NCBI ID   WP_097734995.1    Uniprot ID   -
Organism   Escherichia coli strain UTME-13     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 535814..547247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABH544_RS02575 (ABH544_02575) elbB 536506..537159 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  ABH544_RS02580 (ABH544_02580) mtgA 537156..537884 (+) 729 WP_000047084.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABH544_RS02585 (ABH544_02585) yrbL 537881..538513 (-) 633 WP_000620417.1 PhoP regulatory network protein YrbL -
  ABH544_RS02590 (ABH544_02590) npr 538726..538998 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABH544_RS02595 (ABH544_02595) rapZ 538995..539849 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ABH544_RS02600 (ABH544_02600) ptsN 539895..540386 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABH544_RS02605 (ABH544_02605) hpf 540504..540791 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABH544_RS02610 (ABH544_02610) rpoN/rpoN1 540814..542247 (-) 1434 WP_097734995.1 RNA polymerase factor sigma-54 Machinery gene
  ABH544_RS02615 (ABH544_02615) lptB 542295..543020 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABH544_RS02620 (ABH544_02620) lptA 543027..543584 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABH544_RS02625 (ABH544_02625) lptC 543553..544128 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABH544_RS02630 (ABH544_02630) kdsC 544125..544691 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABH544_RS02635 (ABH544_02635) kdsD 544712..545698 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABH544_RS02640 (ABH544_02640) yrbG 545712..546689 (-) 978 WP_000922861.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53974.74 Da        Isoelectric Point: 4.3637

>NTDB_id=901876 ABH544_RS02610 WP_097734995.1 540814..542247(-) (rpoN/rpoN1) [Escherichia coli strain UTME-13]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIH
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=901876 ABH544_RS02610 WP_097734995.1 540814..542247(-) (rpoN/rpoN1) [Escherichia coli strain UTME-13]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCAC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465