Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SC192_RS15865 Genome accession   NZ_CP138205
Coordinates   3332563..3333363 (-) Length   266 a.a.
NCBI ID   WP_348895605.1    Uniprot ID   A0AB39K440
Organism   Acinetobacter sp. 2019-01-05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3327563..3338363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SC192_RS15845 (SC192_15855) - 3328628..3329326 (-) 699 WP_348895601.1 YfgM family protein -
  SC192_RS15850 (SC192_15860) hisS 3329355..3330647 (-) 1293 WP_348895602.1 histidine--tRNA ligase -
  SC192_RS15855 (SC192_15865) ispG 3330644..3331759 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SC192_RS15860 (SC192_15870) - 3331781..3332572 (-) 792 WP_348895604.1 helix-turn-helix domain-containing protein -
  SC192_RS15865 (SC192_15875) pilF 3332563..3333363 (-) 801 WP_348895605.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SC192_RS15870 (SC192_15880) rlmN 3333379..3334614 (-) 1236 WP_348895606.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SC192_RS15875 (SC192_15885) ndk 3334738..3335169 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  SC192_RS15880 (SC192_15890) iscX 3335283..3335483 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  SC192_RS15885 (SC192_15895) - 3335583..3336134 (-) 552 WP_348895607.1 phosphatase PAP2 family protein -
  SC192_RS15890 (SC192_15900) - 3336159..3337457 (-) 1299 WP_348895686.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30120.48 Da        Isoelectric Point: 9.0211

>NTDB_id=901408 SC192_RS15865 WP_348895605.1 3332563..3333363(-) (pilF) [Acinetobacter sp. 2019-01-05]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVHTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=901408 SC192_RS15865 WP_348895605.1 3332563..3333363(-) (pilF) [Acinetobacter sp. 2019-01-05]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCTGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACGCTTAAAAAGGACCCAGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCTGAATACATTCGTTCTGGTGACTTGG
ACTCTGCAAAACGGTCCCTTGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACAGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGACAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTATCAAATGGAGCGTTATAATGATGCCATCGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGACCAACGCTATCAGGCTTTAGAAAATCTAGGACGGATTTATTTAAAGTTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTTCTTGCTAACCGCGATTCCTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATACGTTCATACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-205)

Tetratricopeptide repeat (TPR_16)

(89-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726