Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RA179_RS13185 Genome accession   NZ_CP138201
Coordinates   2578361..2578786 (-) Length   141 a.a.
NCBI ID   WP_095713328.1    Uniprot ID   -
Organism   Bacillus halotolerans strain DY299     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2573361..2583786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA179_RS13155 (RA179_13155) - 2573600..2573734 (-) 135 WP_010335019.1 hypothetical protein -
  RA179_RS13160 (RA179_13160) - 2573812..2574543 (-) 732 WP_101864558.1 SGNH/GDSL hydrolase family protein -
  RA179_RS13165 (RA179_13165) - 2574790..2575542 (-) 753 WP_024122121.1 N-acetylmuramoyl-L-alanine amidase -
  RA179_RS13170 (RA179_13170) - 2575737..2576363 (+) 627 WP_024122122.1 TVP38/TMEM64 family protein -
  RA179_RS13175 (RA179_13175) gnd 2576385..2577278 (-) 894 WP_106020128.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  RA179_RS13180 (RA179_13180) - 2577599..2578327 (+) 729 WP_106020129.1 hypothetical protein -
  RA179_RS13185 (RA179_13185) nucA/comI 2578361..2578786 (-) 426 WP_095713328.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RA179_RS13190 (RA179_13190) sigK 2578963..2579691 (+) 729 WP_174754429.1 RNA polymerase sporulation sigma factor SigK -
  RA179_RS13195 (RA179_13195) - 2579828..2580109 (-) 282 Protein_2549 recombinase family protein -
  RA179_RS13200 (RA179_13200) - 2580024..2580203 (-) 180 WP_318751889.1 hypothetical protein -
  RA179_RS13205 (RA179_13205) - 2580577..2581026 (+) 450 WP_318751890.1 DUF1569 domain-containing protein -
  RA179_RS13210 (RA179_13210) - 2581859..2582416 (-) 558 WP_101861070.1 XRE family transcriptional regulator -
  RA179_RS13215 (RA179_13215) wzy 2582560..2583777 (+) 1218 WP_106020130.1 O-antigen polysaccharide polymerase Wzy -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15496.62 Da        Isoelectric Point: 5.2217

>NTDB_id=901358 RA179_RS13185 WP_095713328.1 2578361..2578786(-) (nucA/comI) [Bacillus halotolerans strain DY299]
MKRWMAMLLLAAAVFLGLLGKDMTAPQQVKGAALYDKVLYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=901358 RA179_RS13185 WP_095713328.1 2578361..2578786(-) (nucA/comI) [Bacillus halotolerans strain DY299]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCAGTTTTTCTTGGTTTATTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAGTTTTATATTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAATCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGTGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCAGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTGA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.905

89.362

0.553