Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACE1SL_RS02045 Genome accession   NZ_AP027769
Coordinates   445419..446096 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 2017.03.02CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440419..451096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1SL_RS02030 (VEE52_04060) chiA 441786..444479 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  ACE1SL_RS02035 (VEE52_04070) bfd 444648..444842 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  ACE1SL_RS02040 (VEE52_04080) bfr 444914..445390 (+) 477 WP_000675504.1 bacterioferritin -
  ACE1SL_RS02045 (VEE52_04090) pilD 445419..446096 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  ACE1SL_RS02050 (VEE52_04100) gspM 446096..446557 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  ACE1SL_RS02055 (VEE52_04110) gspL 446554..447717 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  ACE1SL_RS02060 (VEE52_04120) gspK 447732..448715 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  ACE1SL_RS02065 (VEE52_04130) gspJ 448708..449295 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  ACE1SL_RS02070 (VEE52_04140) gspI 449288..449665 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  ACE1SL_RS02075 (VEE52_04150) gspH 449662..450171 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  ACE1SL_RS02080 (VEE52_04160) gspG 450179..450616 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=90125 ACE1SL_RS02045 WP_000178161.1 445419..446096(-) (pilD) [Escherichia coli strain 2017.03.02CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=90125 ACE1SL_RS02045 WP_000178161.1 445419..446096(-) (pilD) [Escherichia coli strain 2017.03.02CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTGCC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378