Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   R2B76_RS20785 Genome accession   NZ_CP137800
Coordinates   3956106..3956480 (+) Length   124 a.a.
NCBI ID   WP_318618865.1    Uniprot ID   -
Organism   Priestia megaterium strain Y18-01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3951106..3961480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2B76_RS20765 (R2B76_20775) - 3951596..3952267 (-) 672 WP_057242881.1 response regulator transcription factor -
  R2B76_RS20770 (R2B76_20780) - 3952593..3953411 (+) 819 WP_075419317.1 phytoene/squalene synthase family protein -
  R2B76_RS20775 (R2B76_20785) - 3953447..3955396 (-) 1950 WP_318615408.1 FUSC family protein -
  R2B76_RS20780 (R2B76_20790) - 3955553..3955813 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  R2B76_RS20785 (R2B76_20795) nucA/comI 3956106..3956480 (+) 375 WP_318618865.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  R2B76_RS20790 (R2B76_20800) comJ 3956516..3956923 (+) 408 WP_075419320.1 competence protein ComJ -
  R2B76_RS20795 (R2B76_20805) helD 3956952..3959270 (-) 2319 WP_318615410.1 RNA polymerase recycling motor HelD -
  R2B76_RS20800 (R2B76_20810) - 3959514..3959639 (-) 126 WP_016765178.1 hypothetical protein -
  R2B76_RS20805 (R2B76_20815) - 3959779..3960294 (-) 516 WP_075419322.1 enoyl-CoA hydratase/isomerase family protein -
  R2B76_RS20810 (R2B76_20820) - 3960537..3960905 (+) 369 WP_013084552.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13677.90 Da        Isoelectric Point: 4.2601

>NTDB_id=900726 R2B76_RS20785 WP_318618865.1 3956106..3956480(+) (nucA/comI) [Priestia megaterium strain Y18-01]
MGHAGFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTSYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=900726 R2B76_RS20785 WP_318618865.1 3956106..3956480(+) (nucA/comI) [Priestia megaterium strain Y18-01]
ATGGGACATGCGGGTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTATGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCGGAAACGGGTGATCATATTCGTGACGCAATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGTGAAGAATCGCTGAAAGGATATCCGACAAAAACAAGTTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGCGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.387

95.968

0.637