Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   R2B76_RS17325 Genome accession   NZ_CP137800
Coordinates   3359772..3360194 (+) Length   140 a.a.
NCBI ID   WP_013083984.1    Uniprot ID   A0A1Q8UP88
Organism   Priestia megaterium strain Y18-01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3354772..3365194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2B76_RS17300 (R2B76_17305) - 3355115..3355948 (-) 834 WP_075421018.1 AraC family transcriptional regulator -
  R2B76_RS17305 (R2B76_17310) - 3356172..3356993 (+) 822 WP_318614554.1 histidine kinase -
  R2B76_RS17310 (R2B76_17315) - 3356994..3357635 (+) 642 WP_053487482.1 response regulator transcription factor -
  R2B76_RS17315 (R2B76_17320) - 3357766..3359148 (+) 1383 WP_318614561.1 PepSY domain-containing protein -
  R2B76_RS17320 (R2B76_17325) - 3359178..3359642 (+) 465 WP_075421021.1 FixH family protein -
  R2B76_RS17325 (R2B76_17330) nucA/comI 3359772..3360194 (+) 423 WP_013083984.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  R2B76_RS17330 (R2B76_17335) - 3360349..3360702 (+) 354 WP_234943443.1 DUF1516 family protein -
  R2B76_RS17335 (R2B76_17340) - 3360743..3361336 (-) 594 WP_318614565.1 hypothetical protein -
  R2B76_RS17340 (R2B76_17345) - 3361610..3362095 (-) 486 WP_251530217.1 hypothetical protein -
  R2B76_RS17345 (R2B76_17350) fsa 3362174..3362842 (-) 669 WP_075421024.1 fructose-6-phosphate aldolase -
  R2B76_RS17350 (R2B76_17355) gnd 3362892..3363785 (-) 894 WP_013057957.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15356.20 Da        Isoelectric Point: 4.4990

>NTDB_id=900721 R2B76_RS17325 WP_013083984.1 3359772..3360194(+) (nucA/comI) [Priestia megaterium strain Y18-01]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGNGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=900721 R2B76_RS17325 WP_013083984.1 3359772..3360194(+) (nucA/comI) [Priestia megaterium strain Y18-01]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGTGCTGAAGAAAACCGCCGTGAATCTTTAAAA
GGCATTCCAACTAAAAGCGGCTACGATCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAACGGTGCAGACAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGATCTTGGATCTCACATCAAGTAGACGAGTTTCCAGACGGCACGA
AGGTTTTAATTGAAGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q8UP88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507