Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ99_RS02210 Genome accession   NZ_AP027764
Coordinates   482827..483504 (-) Length   225 a.a.
NCBI ID   WP_039021458.1    Uniprot ID   -
Organism   Escherichia coli strain 2017-02-2CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477827..488504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ99_RS02195 (VEE76_04260) chiA 479194..481887 (+) 2694 WP_115739460.1 bifunctional chitinase/lysozyme -
  AABJ99_RS02200 (VEE76_04270) bfd 482056..482250 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABJ99_RS02205 (VEE76_04280) bfr 482322..482798 (+) 477 WP_000675505.1 bacterioferritin -
  AABJ99_RS02210 (VEE76_04290) pilD 482827..483504 (-) 678 WP_039021458.1 prepilin peptidase GspO Machinery gene
  AABJ99_RS02215 (VEE76_04300) gspM 483504..483965 (-) 462 WP_039021459.1 type II secretion system protein GspM -
  AABJ99_RS02220 (VEE76_04310) gspL 483962..485125 (-) 1164 WP_039021460.1 type II secretion system protein GspL -
  AABJ99_RS02225 (VEE76_04320) gspK 485140..486123 (-) 984 WP_039021461.1 type II secretion system minor pseudopilin GspK -
  AABJ99_RS02230 (VEE76_04330) gspJ 486116..486703 (-) 588 WP_039021462.1 type II secretion system minor pseudopilin GspJ -
  AABJ99_RS02235 (VEE76_04340) gspI 486696..487073 (-) 378 WP_039021463.1 type II secretion system minor pseudopilin GspI -
  AABJ99_RS02240 (VEE76_04350) gspH 487070..487579 (-) 510 WP_039021464.1 type II secretion system minor pseudopilin GspH -
  AABJ99_RS02245 (VEE76_04360) gspG 487587..488024 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24840.72 Da        Isoelectric Point: 7.9585

>NTDB_id=90044 AABJ99_RS02210 WP_039021458.1 482827..483504(-) (pilD) [Escherichia coli strain 2017-02-2CC]
MTMLLPLFILLGFIAGYFVNAMAYHLSPLEDKTALTFRQVLIHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=90044 AABJ99_RS02210 WP_039021458.1 482827..483504(-) (pilD) [Escherichia coli strain 2017-02-2CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGCTTGGTTTTATAGCAGGTTATTTTGTTAATGCTATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGATTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTAACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCACTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCGTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.721

100

0.387

Multiple sequence alignment