Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABGV14_RS16535 Genome accession   NZ_CP156813
Coordinates   3386820..3388253 (-) Length   477 a.a.
NCBI ID   WP_105075472.1    Uniprot ID   -
Organism   Escherichia coli strain PKF8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3381820..3393253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABGV14_RS16500 elbB 3382511..3383164 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  ABGV14_RS16505 mtgA 3383161..3383889 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABGV14_RS16510 yrbL 3383886..3384518 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ABGV14_RS16515 npr 3384732..3385004 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABGV14_RS16520 rapZ 3385001..3385855 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ABGV14_RS16525 ptsN 3385901..3386392 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABGV14_RS16530 hpf 3386510..3386797 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABGV14_RS16535 rpoN/rpoN1 3386820..3388253 (-) 1434 WP_105075472.1 RNA polymerase factor sigma-54 Machinery gene
  ABGV14_RS16540 lptB 3388301..3389026 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABGV14_RS16545 lptA 3389033..3389590 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABGV14_RS16550 lptC 3389559..3390134 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABGV14_RS16555 kdsC 3390131..3390697 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABGV14_RS16560 kdsD 3390718..3391704 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABGV14_RS16565 yrbG 3391718..3392695 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53917.72 Da        Isoelectric Point: 4.3837

>NTDB_id=900223 ABGV14_RS16535 WP_105075472.1 3386820..3388253(-) (rpoN/rpoN1) [Escherichia coli strain PKF8]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSGQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=900223 ABGV14_RS16535 WP_105075472.1 3386820..3388253(-) (rpoN/rpoN1) [Escherichia coli strain PKF8]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGGACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.825

100

0.461