Detailed information NTG0899573

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: R5P05_RS02225
• Genome accession: NZ_CP137632
• Coordinates: 400353..401297 (-)
• Length: 314 a.a.
• NCBI ID: WP_053951809.1
• Uniprot ID: A0A0M4NXN7
• Organism: Candidatus Thioglobus autotrophicus strain EF2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 395353..406297

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5P05_RS02195 (R5P05_02195)	-	396047..396328 (+)	282	WP_318844044.1	4a-hydroxytetrahydrobiopterin dehydratase	-
R5P05_RS02200 (R5P05_02200)	-	396332..397009 (+)	678	WP_318844045.1	hypothetical protein	-
R5P05_RS02205 (R5P05_02205)	-	397011..398000 (+)	990	WP_053951805.1	zinc-binding alcohol dehydrogenase family protein	-
R5P05_RS02210 (R5P05_02210)	-	398036..398515 (-)	480	WP_318844947.1	hypothetical protein	-
R5P05_RS02215 (R5P05_02215)	-	398505..399752 (-)	1248	WP_318844948.1	TolC family protein	-
R5P05_RS02220 (R5P05_02220)	-	399749..400246 (-)	498	WP_082319654.1	thioredoxin fold domain-containing protein	-
R5P05_RS02225 (R5P05_02225)	pilA	400353..401297 (-)	945	WP_053951809.1	signal recognition particle-docking protein FtsY	Machinery gene
R5P05_RS02230 (R5P05_02230)	-	401323..402654 (+)	1332	WP_318844949.1	pitrilysin family protein	-
R5P05_RS02235 (R5P05_02235)	-	402651..403943 (+)	1293	WP_318844950.1	pitrilysin family protein	-
R5P05_RS02240 (R5P05_02240)	-	404046..404636 (+)	591	WP_318844951.1	rhodanese-like domain-containing protein	-
R5P05_RS02245 (R5P05_02245)	-	404746..405453 (+)	708	WP_053951812.1	rhodanese-like domain-containing protein	-

Sequence

Protein

• Length: 314 a.a.
• Molecular weight: 34102.03 Da
• Isoelectric Point: 8.3243

>NTDB_id=899573 R5P05_RS02225 WP_053951809.1 400353..401297(-) (pilA) [Candidatus Thioglobus autotrophicus strain EF2]
MFNFFKKDKSDPIEKSTSLKERLAKSRQKLGSGLSSLLLGKKAIDEDLLDELETLLITADIGISTTDKVLESVRKNASRK
ILKDSDSLYQFLKDELSELLIKDSQLNTDTNETFVILVVGINGAGKTTTIGKLAKSFQNQGKSVMLAAGDTFRAAAVEQL
KVWGERNEIPVVAQTTGSDAASVIYDAYESARAKNIDILIADTAGRLHTQGNLMQELGKIKRVLKKHNENAPHETMLVID
GGSGQNAINQAKEFNKTVGLSGISITKLDGTAKGGVVFAISDELKLPVRYIGVGEGINDLKPFNAKKFVDALFE

Nucleotide

• Length: 945 bp

>NTDB_id=899573 R5P05_RS02225 WP_053951809.1 400353..401297(-) (pilA) [Candidatus Thioglobus autotrophicus strain EF2]
TTGTTTAATTTTTTTAAAAAAGATAAATCTGACCCAATAGAAAAATCCACCTCGCTAAAAGAGCGCCTGGCCAAATCTAG
GCAAAAATTAGGCTCGGGATTATCCAGCTTACTATTAGGTAAAAAAGCCATTGATGAGGATTTGCTAGATGAGCTGGAAA
CGCTCCTAATCACAGCAGATATTGGCATTAGCACTACAGACAAAGTGCTTGAATCGGTACGCAAAAACGCCTCACGAAAA
ATACTAAAAGACTCTGATAGTTTGTATCAATTCTTAAAAGATGAATTATCTGAATTACTGATTAAAGACAGCCAATTAAA
TACCGATACCAACGAAACATTTGTGATTTTAGTAGTGGGTATTAATGGTGCTGGCAAAACCACCACCATCGGCAAACTGG
CCAAATCTTTTCAAAATCAAGGCAAGTCTGTCATGCTCGCAGCAGGCGATACTTTTCGCGCCGCCGCCGTTGAACAGCTA
AAAGTATGGGGTGAGCGCAACGAAATACCAGTTGTTGCTCAAACAACTGGCTCTGACGCCGCCTCGGTGATTTACGATGC
CTATGAATCAGCACGGGCTAAAAATATTGATATTCTTATTGCTGATACCGCAGGACGGTTGCATACTCAAGGTAATCTAA
TGCAAGAGCTTGGAAAAATAAAGCGCGTACTAAAAAAACACAATGAAAATGCCCCACATGAAACCATGCTGGTTATTGAT
GGCGGCTCGGGGCAAAACGCCATTAATCAGGCCAAAGAGTTTAACAAGACAGTTGGACTAAGCGGCATTAGTATCACCAA
GCTTGATGGCACTGCTAAAGGTGGTGTGGTATTTGCTATTTCTGATGAGCTTAAATTGCCCGTACGCTATATTGGTGTTG
GTGAAGGGATTAATGATTTAAAGCCTTTTAACGCTAAAAAGTTTGTCGACGCATTGTTTGAGTAG

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (30-96)

• SRP54-type protein, GTPase domain (SRP54) (114-313)

3D structure

• AlphaFold DB: A0A0M4NXN7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	54.153	95.86	0.519