Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CBOS2020_RS17375 Genome accession   NZ_AP025140
Coordinates   3694449..3695807 (-) Length   452 a.a.
NCBI ID   WP_003360170.1    Uniprot ID   -
Organism   Clostridium botulinum strain Osaka2020     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3689449..3700807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBOS2020_RS17355 (CBOS2020_34290) ispD 3690743..3691432 (-) 690 WP_003360173.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CBOS2020_RS17360 (CBOS2020_34300) - 3691434..3692534 (-) 1101 WP_003360172.1 PIN/TRAM domain-containing protein -
  CBOS2020_RS17365 (CBOS2020_34310) - 3692939..3693337 (+) 399 WP_003357596.1 hypothetical protein -
  CBOS2020_RS17370 (CBOS2020_34320) disA 3693369..3694430 (-) 1062 WP_003360171.1 DNA integrity scanning diadenylate cyclase DisA -
  CBOS2020_RS17375 (CBOS2020_34330) radA 3694449..3695807 (-) 1359 WP_003360170.1 DNA repair protein RadA Machinery gene
  CBOS2020_RS17380 (CBOS2020_34340) - 3695928..3698363 (-) 2436 WP_003360169.1 ATP-dependent Clp protease ATP-binding subunit -
  CBOS2020_RS17385 (CBOS2020_34350) - 3698365..3699408 (-) 1044 WP_003360168.1 protein arginine kinase -
  CBOS2020_RS17390 (CBOS2020_34360) - 3699413..3699940 (-) 528 WP_003360167.1 UvrB/UvrC motif-containing protein -
  CBOS2020_RS17395 (CBOS2020_34370) - 3699963..3700427 (-) 465 WP_003360166.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49783.77 Da        Isoelectric Point: 8.1297

>NTDB_id=89935 CBOS2020_RS17375 WP_003360170.1 3694449..3695807(-) (radA) [Clostridium botulinum strain Osaka2020]
MAKNKIVYICQQCGYESIKWLGKCPGCNSWNSMVEEEKQSTNKTIKNINIKSEPKIISTIKSSEYERLDTGINELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIATKYGKILYVSGEESEEQIKMRADRLKVISKDIYILSETNVDIIKEHITT
INPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMLISKSNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGTRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNIPFYNCDVYVNVVGGLDLTGTFADFGLALALLSSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTNIIIPDRNKDKISVKNNVNLIGISSLREGINKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=89935 CBOS2020_RS17375 WP_003360170.1 3694449..3695807(-) (radA) [Clostridium botulinum strain Osaka2020]
ATGGCTAAAAATAAAATTGTTTATATATGTCAACAATGTGGATATGAATCGATAAAATGGTTAGGAAAATGTCCAGGATG
CAATTCGTGGAATAGTATGGTAGAGGAAGAAAAGCAATCAACTAATAAGACTATAAAAAATATAAATATTAAATCAGAGC
CTAAAATAATATCTACAATAAAATCAAGTGAATATGAGAGATTAGATACTGGAATAAATGAACTGAATAGAGTCTTAGGC
GGTGGCATAGTTAAGGGATCTTTAACTCTTATATCTGGATCGCCTGGCATAGGGAAGTCTACAATACTTTTACAAGCTGC
TAATAATATTGCTACTAAGTATGGTAAGATTTTATATGTGTCTGGAGAAGAATCAGAAGAGCAGATAAAAATGAGAGCAG
ATAGGCTTAAGGTGATATCAAAAGATATATACATACTTTCAGAGACAAATGTGGATATAATAAAAGAGCATATTACTACA
ATTAATCCTAAATTTGTTATAATAGATTCTATACAGACCCTTTTTAAAAGTGAACTATCTTCAGCTCCAGGTACTGTGTC
TCAAGTTAGGCAATGTTCTAACGATATTATGCTTATAAGTAAAAGCAATAACATACCTTTTTTTATAGTAGCACATGTTA
CAAAACAAGGAGAATTAGCAGGACCTAGGGTATTAGAACATATGGTAGATACGGTTTTATCATTTGAAGGAGAAAGAACC
CAGGAATTTAGGATTTTAAGAACAGTAAAAAATCGTTTTGGAACTACAAGTGAAATTGGAGTTTTTGAAATGGCAGGAGA
AGGGCTATTAGAAATAAGCAATCCCTCTGCTGTATTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGGACTAGGCCAATACTTATAGAAATACAAGCATTGGTAAGTGAAACGAAAGCTTATATGCCAAGAAGG
ACGGCAGTGGGAGTAGACACAGCTAGATTGAATTTAATATTAGCTGTGTTAGAAAAAAAATTAAATATACCTTTTTATAA
TTGTGATGTATATGTTAATGTAGTAGGAGGATTAGATTTAACAGGAACTTTTGCTGATTTCGGATTAGCATTAGCTTTAT
TATCTAGTGCTAAATCAAAAGATATAAAGTTACCTAAAGTTTTAGTGGTTGGAGAAATAGGACTTACAGGAGAGGTTCGC
CCCGTAAGTTTTTGTGATAGAATAGTAAATGAAGGTATAAAAATGGGATTTACCAATATAATTATACCTGATAGAAATAA
AGACAAAATTTCTGTTAAAAATAATGTTAACCTAATTGGTATATCTTCTTTAAGAGAAGGTATTAATAAAGTATTTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-217)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.89

100

0.5
  radA Streptococcus pneumoniae Rx1

48.678

100

0.489
  radA Streptococcus pneumoniae D39

48.678

100

0.489
  radA Streptococcus pneumoniae R6

48.678

100

0.489
  radA Streptococcus pneumoniae TIGR4

48.678

100

0.489
  radA Streptococcus mitis NCTC 12261

48.678

100

0.489
  radA Streptococcus mitis SK321

48.678

100

0.489

Multiple sequence alignment