Detailed information NTG0899118

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R6U47_RS22490
• Genome accession: NZ_CP137561
• Coordinates: 4769966..4771114 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CPA0053
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4764966..4776114

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6U47_RS22470 (R6U47_22475)	-	4765704..4766888 (-)	1185	WP_023086800.1	cystathionine gamma-synthase	-
R6U47_RS22475 (R6U47_22480)	-	4766885..4768258 (-)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
R6U47_RS22480 (R6U47_22485)	-	4768501..4768905 (-)	405	WP_003084560.1	NINE protein	-
R6U47_RS22485 (R6U47_22490)	czcD	4769059..4769958 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
R6U47_RS22490 (R6U47_22495)	pilU	4769966..4771114 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R6U47_RS22495 (R6U47_22500)	pilT	4771292..4772326 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R6U47_RS22500 (R6U47_22505)	-	4772541..4773233 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R6U47_RS22505 (R6U47_22510)	proC	4773245..4774066 (+)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
R6U47_RS22510 (R6U47_22515)	-	4774077..4774670 (+)	594	WP_003084544.1	YggT family protein	-
R6U47_RS22515 (R6U47_22520)	-	4774667..4774963 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=899118 R6U47_RS22490 WP_003100959.1 4769966..4771114(-) (pilU) [Pseudomonas aeruginosa strain CPA0053]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=899118 R6U47_RS22490 WP_003100959.1 4769966..4771114(-) (pilU) [Pseudomonas aeruginosa strain CPA0053]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382