Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACE1SF_RS12290 Genome accession   NZ_AP027754
Coordinates   2507219..2507896 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 2017.02.04CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2502219..2512896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1SF_RS12255 (VEE51_24040) gspI 2502314..2502691 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  ACE1SF_RS12260 (VEE51_24050) gspJ 2502684..2503271 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  ACE1SF_RS12265 (VEE51_24060) gspK 2503264..2504022 (+) 759 Protein_2395 type II secretion system minor pseudopilin GspK -
  ACE1SF_RS12270 (VEE51_24070) - 2504035..2505263 (-) 1229 Protein_2396 IS3 family transposase -
  ACE1SF_RS12275 (VEE51_24100) - 2505356..2505583 (+) 228 Protein_2397 type II secretion system protein K -
  ACE1SF_RS12280 (VEE51_24110) gspL 2505598..2506761 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  ACE1SF_RS12285 (VEE51_24120) gspM 2506758..2507219 (+) 462 WP_032187646.1 type II secretion system protein GspM -
  ACE1SF_RS12290 (VEE51_24130) pilD 2507219..2507896 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  ACE1SF_RS12295 (VEE51_24140) bfr 2507925..2508401 (-) 477 WP_000675504.1 bacterioferritin -
  ACE1SF_RS12300 (VEE51_24150) bfd 2508473..2508667 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  ACE1SF_RS12305 chiA 2508836..2511529 (-) 2694 Protein_2403 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=89909 ACE1SF_RS12290 WP_000178161.1 2507219..2507896(+) (pilD) [Escherichia coli strain 2017.02.04CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=89909 ACE1SF_RS12290 WP_000178161.1 2507219..2507896(+) (pilD) [Escherichia coli strain 2017.02.04CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment