Detailed information NTG0898740

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5029_RS01795
• Genome accession: NZ_CP137522
• Coordinates: 378070..379218 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA0118
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 373070..384218

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5029_RS01770 (R5029_01770)	-	374221..374517 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
R5029_RS01775 (R5029_01775)	-	374514..375107 (-)	594	WP_003084544.1	YggT family protein	-
R5029_RS01780 (R5029_01780)	proC	375118..375939 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5029_RS01785 (R5029_01785)	-	375951..376643 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5029_RS01790 (R5029_01790)	pilT	376858..377892 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5029_RS01795 (R5029_01795)	pilU	378070..379218 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5029_RS01800 (R5029_01800)	czcD	379226..380125 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
R5029_RS01805 (R5029_01805)	-	380280..380684 (+)	405	WP_003084560.1	NINE protein	-
R5029_RS01810 (R5029_01810)	-	380927..382300 (+)	1374	WP_126892857.1	pyridoxal-phosphate dependent enzyme	-
R5029_RS01815 (R5029_01815)	-	382297..383481 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=898740 R5029_RS01795 WP_003100959.1 378070..379218(+) (pilU) [Pseudomonas aeruginosa strain HPA0118]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898740 R5029_RS01795 WP_003100959.1 378070..379218(+) (pilU) [Pseudomonas aeruginosa strain HPA0118]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382