Detailed information NTG0898545

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5023_RS07535
• Genome accession: NZ_CP137504
• Coordinates: 1624112..1625260 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain HPA0124
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1619112..1630260

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5023_RS07510 (R5023_07510)	-	1620263..1620559 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
R5023_RS07515 (R5023_07515)	-	1620556..1621149 (-)	594	WP_003084544.1	YggT family protein	-
R5023_RS07520 (R5023_07520)	proC	1621160..1621981 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5023_RS07525 (R5023_07525)	-	1621993..1622685 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5023_RS07530 (R5023_07530)	pilT	1622900..1623934 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5023_RS07535 (R5023_07535)	pilU	1624112..1625260 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
R5023_RS07540 (R5023_07540)	czcD	1625268..1626167 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
R5023_RS07545 (R5023_07545)	-	1626322..1626726 (+)	405	WP_003084560.1	NINE protein	-
R5023_RS07550 (R5023_07550)	-	1626969..1628342 (+)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
R5023_RS07555 (R5023_07555)	-	1628339..1629523 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=898545 R5023_RS07535 WP_003110016.1 1624112..1625260(+) (pilU) [Pseudomonas aeruginosa strain HPA0124]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898545 R5023_RS07535 WP_003110016.1 1624112..1625260(+) (pilU) [Pseudomonas aeruginosa strain HPA0124]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382