Detailed information NTG0898505

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5020_RS04135
• Genome accession: NZ_CP137500
• Coordinates: 851893..853041 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA0384
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 846893..858041

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5020_RS04115 (R5020_04115)	-	847630..848814 (-)	1185	WP_003084564.1	cystathionine gamma-synthase	-
R5020_RS04120 (R5020_04120)	-	848811..850184 (-)	1374	WP_126892857.1	pyridoxal-phosphate dependent enzyme	-
R5020_RS04125 (R5020_04125)	-	850427..850831 (-)	405	WP_003084560.1	NINE protein	-
R5020_RS04130 (R5020_04130)	czcD	850986..851885 (+)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
R5020_RS04135 (R5020_04135)	pilU	851893..853041 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5020_RS04140 (R5020_04140)	pilT	853219..854253 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5020_RS04145 (R5020_04145)	-	854468..855160 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5020_RS04150 (R5020_04150)	proC	855172..855993 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5020_RS04155 (R5020_04155)	-	856004..856597 (+)	594	WP_003084544.1	YggT family protein	-
R5020_RS04160 (R5020_04160)	-	856594..856890 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=898505 R5020_RS04135 WP_003100959.1 851893..853041(-) (pilU) [Pseudomonas aeruginosa strain HPA0384]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898505 R5020_RS04135 WP_003100959.1 851893..853041(-) (pilU) [Pseudomonas aeruginosa strain HPA0384]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382