Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   R5H20_RS15015 Genome accession   NZ_CP137345
Coordinates   2954163..2955200 (-) Length   345 a.a.
NCBI ID   WP_006637539.1    Uniprot ID   -
Organism   Bacillus sp. KICET-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2949163..2960200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5H20_RS14965 (R5H20_14965) tasA 2949173..2949967 (-) 795 WP_006637529.1 biofilm matrix protein TasA -
  R5H20_RS14970 (R5H20_14970) sipW 2950037..2950618 (-) 582 WP_006637530.1 signal peptidase I SipW -
  R5H20_RS14975 (R5H20_14975) tapA 2950615..2951361 (-) 747 WP_006637531.1 amyloid fiber anchoring/assembly protein TapA -
  R5H20_RS14980 (R5H20_14980) - 2951624..2951944 (+) 321 WP_006637532.1 DUF3889 domain-containing protein -
  R5H20_RS14985 (R5H20_14985) - 2952038..2952223 (-) 186 WP_006637533.1 YqzE family protein -
  R5H20_RS14990 (R5H20_14990) comGG 2952303..2952626 (-) 324 WP_224254418.1 competence type IV pilus minor pilin ComGG -
  R5H20_RS14995 (R5H20_14995) comGF 2952681..2953172 (-) 492 WP_224254419.1 competence type IV pilus minor pilin ComGF -
  R5H20_RS15000 (R5H20_15000) comGE 2953081..2953428 (-) 348 WP_006637536.1 competence type IV pilus minor pilin ComGE -
  R5H20_RS15005 (R5H20_15005) comGD 2953412..2953855 (-) 444 WP_006637537.1 competence type IV pilus minor pilin ComGD -
  R5H20_RS15010 (R5H20_15010) comGC 2953855..2954148 (-) 294 WP_006637538.1 competence type IV pilus major pilin ComGC Machinery gene
  R5H20_RS15015 (R5H20_15015) comGB 2954163..2955200 (-) 1038 WP_006637539.1 competence type IV pilus assembly protein ComGB Machinery gene
  R5H20_RS15020 (R5H20_15020) comGA 2955187..2956254 (-) 1068 WP_006637540.1 competence type IV pilus ATPase ComGA Machinery gene
  R5H20_RS15025 (R5H20_15025) - 2956393..2957250 (-) 858 WP_006637541.1 STAS domain-containing protein -
  R5H20_RS15035 (R5H20_15035) - 2957469..2957849 (-) 381 WP_006637542.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  R5H20_RS15040 (R5H20_15040) - 2958062..2958307 (+) 246 WP_006637543.1 DUF2626 domain-containing protein -
  R5H20_RS15045 (R5H20_15045) - 2958357..2958995 (-) 639 WP_006637544.1 MBL fold metallo-hydrolase -
  R5H20_RS15050 (R5H20_15050) - 2959136..2959309 (+) 174 WP_006637545.1 DUF2759 domain-containing protein -
  R5H20_RS15055 (R5H20_15055) - 2959367..2959681 (-) 315 WP_006637546.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 40024.46 Da        Isoelectric Point: 9.6575

>NTDB_id=897593 R5H20_RS15015 WP_006637539.1 2954163..2955200(-) (comGB) [Bacillus sp. KICET-3]
MKPIKNRWPVGEQAEFLEKLGEMMMNGYTLLDALSMLELQLKRQQKTDIAFGRRKLAEGYPVFQVLNMISFHKAAVSIVY
FAERHGNVPFAFMQSGELLRRKIEQAEKIKKAAHYPAFLILTVCLIVYMMKAAIVPQFSAIYDSMNIETPFLTSFIFLFF
ESFSLLFLCILAAAAVFWAYYLYAFRQKPPEDKMALLIRIPLAGRILKLFNSYFLSLQLSNLLTSGLSIYDSLKAFESQP
FLPFFQKEAKRLIERLKQGEAIEHMLNGHPFYEKDLSKVVAHGQLNGQLHRELYSYSQFLIDRFEKKAEKWTGLLQPLIY
GFTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=897593 R5H20_RS15015 WP_006637539.1 2954163..2955200(-) (comGB) [Bacillus sp. KICET-3]
ATGAAGCCGATTAAGAACAGATGGCCTGTCGGGGAACAGGCAGAGTTCCTTGAAAAGCTTGGCGAGATGATGATGAACGG
TTATACGCTTCTTGATGCATTAAGCATGCTGGAACTGCAATTAAAGCGGCAGCAAAAAACGGATATTGCATTCGGGAGGA
GAAAGCTTGCGGAAGGGTATCCTGTTTTTCAAGTTTTAAATATGATTTCATTTCATAAAGCTGCCGTCAGCATCGTTTAT
TTCGCCGAACGTCACGGTAATGTGCCATTTGCTTTTATGCAGAGCGGCGAATTGCTCCGCCGTAAAATCGAACAGGCCGA
AAAAATCAAAAAAGCCGCACATTATCCGGCATTTTTGATTTTGACGGTTTGCCTCATTGTCTATATGATGAAAGCCGCCA
TTGTTCCGCAGTTTTCCGCGATCTATGACTCGATGAACATAGAAACGCCCTTTCTGACATCCTTCATCTTTTTATTTTTT
GAAAGTTTCTCCTTGTTGTTTCTGTGCATACTAGCCGCCGCTGCTGTTTTTTGGGCGTATTATTTGTACGCTTTCCGCCA
AAAGCCCCCTGAAGACAAAATGGCTCTTCTCATCAGAATTCCGCTGGCAGGCAGAATCCTCAAATTGTTTAACAGCTACT
TTTTATCACTTCAGCTGAGCAATCTTCTTACATCCGGTTTGTCTATATATGACAGTTTAAAAGCGTTTGAAAGCCAGCCC
TTTTTGCCGTTTTTCCAAAAGGAAGCTAAACGGCTGATCGAGAGGCTGAAACAGGGGGAAGCGATAGAACATATGTTAAA
CGGACACCCGTTTTATGAAAAGGACCTATCAAAGGTGGTGGCTCACGGCCAATTAAACGGCCAGCTTCACAGAGAGCTTT
ATTCATACAGCCAATTTCTGATCGACCGGTTTGAAAAGAAAGCGGAAAAGTGGACAGGCCTGCTGCAGCCGCTGATTTAC
GGTTTTACCGCGGCCATGATTTTAATACTCTATCTGTCCATGCTGTTGCCAATGTATCAAATGATGAATCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)

Type II secretion system (T2SS), protein F (T2SSF)

(217-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

56.347

93.623

0.528