Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ81_RS01930 Genome accession   NZ_AP027745
Coordinates   419618..420295 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 2017-01-4CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414618..425295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ81_RS01910 (VEE70_03710) tuf 416005..417189 (+) 1185 WP_000031783.1 elongation factor Tu -
  AABJ81_RS01915 (VEE70_03720) chiA 417383..418678 (+) 1296 Protein_379 bifunctional chitinase/lysozyme -
  AABJ81_RS01920 (VEE70_03730) bfd 418847..419041 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABJ81_RS01925 (VEE70_03740) bfr 419113..419589 (+) 477 WP_000675504.1 bacterioferritin -
  AABJ81_RS01930 (VEE70_03750) pilD 419618..420295 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  AABJ81_RS01935 (VEE70_03760) gspM 420295..420756 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AABJ81_RS01940 (VEE70_03770) gspL 420753..421916 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  AABJ81_RS01945 (VEE70_03780) gspK 421931..422914 (-) 984 WP_124038501.1 type II secretion system minor pseudopilin GspK -
  AABJ81_RS01950 (VEE70_03790) gspJ 422907..423494 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABJ81_RS01955 (VEE70_03800) gspI 423487..423864 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABJ81_RS01960 (VEE70_03810) gspH 423861..424370 (-) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  AABJ81_RS01965 (VEE70_03820) gspG 424378..424815 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=89720 AABJ81_RS01930 WP_000178161.1 419618..420295(-) (pilD) [Escherichia coli strain 2017-01-4CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=89720 AABJ81_RS01930 WP_000178161.1 419618..420295(-) (pilD) [Escherichia coli strain 2017-01-4CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment