Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABD372_RS20425 Genome accession   NZ_CP155528
Coordinates   4164138..4164578 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain 3-72     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4159138..4169578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD372_RS20405 (ABD372_20390) aroP 4160138..4161508 (+) 1371 WP_001540704.1 aromatic amino acid transporter AroP -
  ABD372_RS20410 (ABD372_20395) ampE 4161551..4162405 (-) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  ABD372_RS20415 (ABD372_20400) ampD 4162402..4162953 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABD372_RS20420 (ABD372_20405) nadC 4163041..4163934 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABD372_RS20425 (ABD372_20410) pilA 4164138..4164578 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  ABD372_RS20430 (ABD372_20415) pilB 4164588..4165973 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  ABD372_RS20435 (ABD372_20420) hofC 4165963..4167165 (+) 1203 WP_001540700.1 protein transport protein HofC -
  ABD372_RS20440 (ABD372_20425) guaC 4167200..4168243 (-) 1044 WP_001217338.1 GMP reductase -
  ABD372_RS20445 (ABD372_20430) - 4168399..4168443 (-) 45 WP_120795372.1 protein YacM -
  ABD372_RS20450 (ABD372_20435) coaE 4168468..4169088 (+) 621 WP_001269524.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=896496 ABD372_RS20425 WP_000360914.1 4164138..4164578(+) (pilA) [Escherichia coli strain 3-72]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=896496 ABD372_RS20425 WP_000360914.1 4164138..4164578(+) (pilA) [Escherichia coli strain 3-72]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363