Detailed information NTG0896418

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: R4540_RS00640
• Genome accession: NZ_CP137135
• Coordinates: 133553..134560 (+)
• Length: 335 a.a.
• NCBI ID: WP_000116737.1
• Uniprot ID: O51804
• Organism: Vibrio cholerae V52
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 128553..139560

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4540_RS00605 (R4540_00605)	ftsB	128604..128888 (+)	285	WP_001263189.1	cell division protein FtsB	-
R4540_RS00610 (R4540_00610)	ispD	128885..129583 (+)	699	WP_001061951.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
R4540_RS00615 (R4540_00615)	ispF	129580..130056 (+)	477	WP_000619209.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
R4540_RS00620 (R4540_00620)	truD	130086..131171 (+)	1086	WP_000129752.1	tRNA pseudouridine(13) synthase TruD	-
R4540_RS00625 (R4540_00625)	surE	131173..131925 (+)	753	WP_000698379.1	5'/3'-nucleotidase SurE	-
R4540_RS00630 (R4540_00630)	-	131918..132544 (+)	627	WP_000002982.1	protein-L-isoaspartate(D-aspartate) O-methyltransferase	-
R4540_RS00635 (R4540_00635)	nlpD	132544..133479 (+)	936	WP_000177568.1	murein hydrolase activator NlpD	-
R4540_RS00640 (R4540_00640)	rpoS	133553..134560 (+)	1008	WP_000116737.1	RNA polymerase sigma factor RpoS	Regulator
R4540_RS00645 (R4540_00645)	mutS	134653..137241 (-)	2589	WP_001894770.1	DNA mismatch repair protein MutS	-
R4540_RS00650 (R4540_00650)	cysM	137509..138396 (-)	888	WP_001279365.1	cysteine synthase CysM	-

Sequence

Protein

• Length: 335 a.a.
• Molecular weight: 38534.39 Da
• Isoelectric Point: 4.3648

>NTDB_id=896418 R4540_RS00640 WP_000116737.1 133553..134560(+) (rpoS) [Vibrio cholerae V52]
MSVSNTVTKVEEFDFEDEALEVLETDAELTSDEELVAVEGASEDVREEFDASAKSLDATQMYLSEIGFSPLLTAEEEVLY
ARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALM
NQTRTIRLPIHVVKELNIYLRTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILP
DSHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTRERVRQIQVEGLRRLREILVK
QGLNMEALFNVEYDN

Nucleotide

• Length: 1008 bp

>NTDB_id=896418 R4540_RS00640 WP_000116737.1 133553..134560(+) (rpoS) [Vibrio cholerae V52]
ATGAGTGTCAGCAATACCGTAACCAAAGTAGAAGAGTTCGATTTTGAAGATGAAGCACTGGAAGTGCTAGAAACTGATGC
CGAGCTCACCAGTGATGAAGAATTAGTTGCTGTTGAAGGGGCAAGTGAAGACGTTCGTGAAGAGTTTGATGCTTCTGCGA
AAAGTCTTGATGCGACCCAGATGTATCTCAGCGAAATTGGTTTTTCACCGCTCCTTACTGCCGAAGAAGAAGTGCTTTAT
GCTCGTCGTGCCTTACGTGGTGATGAAGCCGCACGTAAACGCATGATTGAAAGTAACTTGCGTCTGGTGGTAAAAATTTC
ACGCCGTTACAGCAACCGAGGATTAGCACTGCTCGATCTGATTGAAGAAGGTAATCTTGGTCTGATCCGTGCGGTTGAGA
AATTCGATCCAGAACGCGGTTTCCGCTTCTCTACCTACGCAACATGGTGGATCCGTCAAACCATTGAACGTGCGCTGATG
AACCAAACACGCACCATTCGTCTACCGATTCATGTCGTCAAAGAGCTGAACATTTATTTGCGTACTGCTCGCGAATTATC
ACAGCGCCTTGACCACGAACCTACACCAGAAGAAATTGCTCTTGAGTTAGACCGACCTGTCGATGATGTCACTAAGATGC
TGCGTCTTAACGAACGGATCAGCTCAGTGGATACGCCAATTGGTGGGGATGGAGATAAGGCACTGCTGGATATTTTGCCA
GACTCTCACAATGCCGATCCTGAGTTTTCAACTCAAGATGATGACATTCGTGAATCGCTGCTCAACTGGTTGGATGAACT
TAATCCAAAGCAAAAAGAAGTGCTTGCGCGTCGCTTTGGGCTTCTTGGCTATGAACCATCGACCTTGGAAGAAGTGGGTC
GTGAGATCAATCTCACTCGTGAGCGTGTTCGCCAAATCCAAGTGGAAGGTCTACGTCGTCTGCGTGAGATTTTGGTGAAA
CAAGGTTTGAATATGGAAGCGCTGTTTAACGTCGAATACGACAACTAA

Domains

Predicted by InterproScan.

• Sigma-70, region 4 (Sigma70_r4) (263-316)

• Sigma-70 region 3 (Sigma70_r3) (175-249)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (57-90)

• Sigma-70 region 2 (Sigma70_r2) (95-164)

3D structure

• AlphaFold DB: O51804

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	100	100	1