Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R5L00_RS07805 Genome accession   NZ_CP137134
Coordinates   1757484..1758947 (+) Length   487 a.a.
NCBI ID   WP_317654067.1    Uniprot ID   A0AAU0KPK3
Organism   Nitrosospira sp. Is2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1752484..1763947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5L00_RS07775 (R5L00_07775) - 1752513..1753505 (+) 993 WP_317654065.1 cobalamin-binding protein -
  R5L00_RS07780 (R5L00_07780) ispH 1753745..1754689 (+) 945 WP_107694468.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  R5L00_RS07785 (R5L00_07785) - 1754992..1755516 (+) 525 WP_107694469.1 KdsC family phosphatase -
  R5L00_RS07790 (R5L00_07790) lptC 1755513..1756085 (+) 573 WP_107694470.1 LPS export ABC transporter periplasmic protein LptC -
  R5L00_RS07795 (R5L00_07795) lptA 1756192..1756686 (+) 495 WP_317654144.1 lipopolysaccharide transport periplasmic protein LptA -
  R5L00_RS07800 (R5L00_07800) lptB 1756711..1757433 (+) 723 WP_107694471.1 LPS export ABC transporter ATP-binding protein -
  R5L00_RS07805 (R5L00_07805) rpoN/rpoN1 1757484..1758947 (+) 1464 WP_317654067.1 RNA polymerase factor sigma-54 Machinery gene
  R5L00_RS07810 (R5L00_07810) hpf 1758968..1759324 (+) 357 WP_107694473.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R5L00_RS07815 (R5L00_07815) hprK 1759381..1760349 (+) 969 WP_317654068.1 HPr(Ser) kinase/phosphatase -
  R5L00_RS07820 (R5L00_07820) rapZ 1760391..1761230 (+) 840 WP_317654070.1 RNase adapter RapZ -
  R5L00_RS07825 (R5L00_07825) - 1761277..1761897 (+) 621 WP_317654071.1 flavin prenyltransferase UbiX -
  R5L00_RS07830 (R5L00_07830) - 1761980..1762624 (-) 645 WP_317654072.1 5-formyltetrahydrofolate cyclo-ligase -
  R5L00_RS07835 (R5L00_07835) mutY 1762617..1763723 (-) 1107 WP_317654074.1 A/G-specific adenine glycosylase -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 54542.29 Da        Isoelectric Point: 4.8151

>NTDB_id=896403 R5L00_RS07805 WP_317654067.1 1757484..1758947(+) (rpoN/rpoN1) [Nitrosospira sp. Is2]
MKPTLQLKTSQHLTLTPQLQESIRLLQLSTLELNQEIERLAQENPLLELDDSVGSGIEYLGALEAGVEPLSADLMEIPEE
GGSKLLSEDSLDSKKEAGETDWLPDDSGSRGMRDDDDERDFPQQAAEPQSLREHLNSQLSLSQIPERDRKIVGLLIDSLD
DDGYLAQDLEELVDLLPPELEIDIDDLHIARELLQHLDPAGVGARNLRECLVMQLQALPEDTPYRDQALLLVGNHLESLA
SRDFCAIKKLLHCDDDCLRSIQNLITRLNPRPGMAFSSAVARYIIPDVIVTKIGGVWVANLNPEAMPRLRINRLYADILK
RYSDDSTRRLAGQLNEAKWLIKNVLQRFSTILKVSAAVVERQQQFFEHGPVAMRPMVLREIASTLDLHESTISRVTTQKF
MRTPRGIFELKYFFGSHVATDTGGVCSATAIRALIKQLISAEEGKKPLSDSQISEILGQQGIMVARRTVAKYRESMHIPP
VNLRKSV

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=896403 R5L00_RS07805 WP_317654067.1 1757484..1758947(+) (rpoN/rpoN1) [Nitrosospira sp. Is2]
ATGAAACCCACTCTCCAGCTTAAGACGTCACAGCACTTAACGCTCACTCCGCAATTGCAGGAATCGATCAGGTTGCTGCA
GCTGTCAACGCTGGAGCTGAACCAGGAAATTGAACGCCTGGCTCAGGAAAATCCCCTTCTGGAGCTCGACGACAGTGTCG
GTAGCGGCATCGAGTATTTGGGCGCGCTCGAGGCCGGGGTAGAGCCCCTTTCGGCTGATCTGATGGAAATTCCGGAGGAA
GGCGGCAGCAAATTGCTTTCCGAGGATAGTCTTGACTCAAAAAAAGAAGCGGGGGAAACGGACTGGCTCCCAGACGACAG
CGGATCCCGCGGCATGCGGGACGATGACGACGAGCGTGATTTTCCACAACAGGCGGCAGAGCCTCAAAGTCTGCGCGAGC
ACCTCAACTCGCAGCTTAGCCTTAGTCAGATTCCTGAACGCGACAGGAAAATTGTCGGTTTACTGATCGACAGCCTCGAC
GACGATGGGTACCTTGCCCAGGACCTGGAAGAACTCGTTGACCTGTTGCCGCCCGAACTCGAGATAGACATCGATGACCT
TCACATCGCCCGTGAACTATTACAACATCTCGATCCGGCCGGCGTGGGGGCCCGTAACCTGAGAGAATGCCTGGTGATGC
AGCTGCAGGCTCTGCCGGAGGACACGCCATATCGTGATCAGGCCTTGCTGCTGGTCGGGAATCATCTTGAAAGCCTGGCG
TCAAGAGACTTTTGCGCAATAAAAAAATTGCTGCATTGCGACGACGACTGCTTGCGTTCGATCCAGAATTTGATCACGCG
CCTAAACCCCCGGCCCGGTATGGCATTCAGTTCCGCAGTGGCCCGCTATATTATTCCTGATGTGATTGTCACCAAAATCG
GCGGCGTATGGGTCGCCAATCTTAATCCGGAAGCAATGCCTCGCCTCAGGATCAACCGGCTGTATGCGGACATCCTGAAA
CGCTATAGCGATGACTCTACGCGGCGGCTGGCAGGTCAACTCAATGAAGCAAAATGGCTTATCAAGAATGTCCTTCAGCG
TTTCAGCACCATATTGAAGGTGTCGGCCGCTGTGGTTGAGCGACAGCAGCAGTTCTTCGAGCATGGGCCAGTAGCCATGC
GACCGATGGTATTGCGCGAAATTGCGAGTACGCTCGACCTGCATGAATCCACCATTTCTCGTGTGACAACGCAGAAATTC
ATGCGTACGCCACGTGGTATATTCGAACTCAAGTATTTTTTTGGTAGTCACGTCGCAACCGATACGGGCGGAGTTTGTTC
CGCGACCGCCATTCGCGCTTTGATAAAACAACTGATCAGTGCCGAGGAGGGAAAAAAACCGTTGAGCGACAGTCAAATTT
CGGAAATTCTGGGCCAGCAGGGTATTATGGTTGCTCGTCGCACTGTGGCAAAATACCGGGAGTCCATGCACATCCCTCCG
GTTAATCTTCGCAAGTCAGTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(123-315)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.559

100

0.548