Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LDN68_RS02085 Genome accession   NZ_AP024948
Coordinates   419489..420928 (+) Length   479 a.a.
NCBI ID   WP_041211850.1    Uniprot ID   A0A2L0TRY9
Organism   Aeromonas caviae strain KAM345     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414489..425928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDN68_RS02055 (KAM345_003910) mlaF 415067..415867 (-) 801 WP_029314846.1 phospholipid ABC transporter ATP-binding protein MlaF -
  LDN68_RS02060 (KAM345_003920) - 416074..417069 (+) 996 WP_029314845.1 KpsF/GutQ family sugar-phosphate isomerase -
  LDN68_RS02065 (KAM345_003930) kdsC 417069..417623 (+) 555 WP_049637178.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LDN68_RS02070 (KAM345_003940) lptC 417620..418180 (+) 561 WP_039040473.1 LPS export ABC transporter periplasmic protein LptC -
  LDN68_RS02075 (KAM345_003950) lptA 418167..418685 (+) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  LDN68_RS02080 (KAM345_003960) lptB 418698..419423 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  LDN68_RS02085 (KAM345_003970) rpoN/rpoN1 419489..420928 (+) 1440 WP_041211850.1 RNA polymerase factor sigma-54 Machinery gene
  LDN68_RS02090 (KAM345_003980) hpf 420949..421236 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  LDN68_RS02095 (KAM345_003990) ptsN 421239..421685 (+) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LDN68_RS02100 (KAM345_004000) rapZ 421726..422592 (+) 867 WP_010675869.1 RNase adapter RapZ -
  LDN68_RS02105 (KAM345_004010) npr 422613..422885 (+) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  LDN68_RS02110 (KAM345_004020) - 422901..424805 (-) 1905 WP_049637179.1 BglG family transcription antiterminator -
  LDN68_RS02115 (KAM345_004030) dagF 424908..425648 (-) 741 WP_049637180.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53731.54 Da        Isoelectric Point: 4.3728

>NTDB_id=89602 LDN68_RS02085 WP_041211850.1 419489..420928(+) (rpoN/rpoN1) [Aeromonas caviae strain KAM345]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQAETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=89602 LDN68_RS02085 WP_041211850.1 419489..420928(+) (rpoN/rpoN1) [Aeromonas caviae strain KAM345]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGGCAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACGGCCCAGAGTGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAAGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCGGCCATCGCCCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ATTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TRY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482

Multiple sequence alignment