Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   R4700_RS04015 Genome accession   NZ_CP137112
Coordinates   782153..782977 (-) Length   274 a.a.
NCBI ID   WP_001036791.1    Uniprot ID   A0A0B7L7Z9
Organism   Streptococcus pneumoniae strain 02H2025     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 777153..787977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4700_RS04010 - 780531..781862 (-) 1332 WP_000390039.1 hemolysin family protein -
  R4700_RS04015 endA 782153..782977 (-) 825 WP_001036791.1 DNA-entry nuclease EndA Machinery gene
  R4700_RS04020 - 783016..783204 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  R4700_RS04025 murA 783197..784480 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  R4700_RS04030 - 784504..784734 (-) 231 WP_000250393.1 DUF1146 family protein -
  R4700_RS04035 sepM 784792..785829 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  R4700_RS04040 coaD 785813..786301 (-) 489 WP_001280740.1 pantetheine-phosphate adenylyltransferase -
  R4700_RS04045 rsmD 786291..786830 (-) 540 WP_000706963.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  R4700_RS04050 asnA 786895..787887 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29939.65 Da        Isoelectric Point: 10.1700

>NTDB_id=896019 R4700_RS04015 WP_001036791.1 782153..782977(-) (endA) [Streptococcus pneumoniae strain 02H2025]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=896019 R4700_RS04015 WP_001036791.1 782153..782977(-) (endA) [Streptococcus pneumoniae strain 02H2025]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACCAAGCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7L7Z9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.54

100

0.985
  endA Streptococcus pneumoniae D39

98.54

100

0.985
  endA Streptococcus pneumoniae R6

98.54

100

0.985
  endA Streptococcus pneumoniae TIGR4

98.54

100

0.985