Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4704_RS04545 Genome accession   NZ_CP137109
Coordinates   854994..855935 (-) Length   313 a.a.
NCBI ID   WP_000249556.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 11012     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 849994..860935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4704_RS04505 comYH 850487..851440 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  R4704_RS04510 - 851501..852088 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  R4704_RS04515 comGG/cglG 852225..852638 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4704_RS04520 comGF/cglF 852616..853077 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4704_RS04525 comGE/cglE 853040..853342 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4704_RS04530 comGD/cglD 853305..853709 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4704_RS04535 comGC/cglC 853702..854028 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  R4704_RS04540 comGB/cglB 854030..855046 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4704_RS04545 comGA/cglA/cilD 854994..855935 (-) 942 WP_000249556.1 competence type IV pilus ATPase ComGA Machinery gene
  R4704_RS04550 - 856011..856376 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4704_RS04555 - 856527..857585 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4704_RS04560 nagA 857748..858899 (-) 1152 WP_033705218.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4704_RS04565 - 859052..860869 (-) 1818 WP_001220843.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35534.46 Da        Isoelectric Point: 6.0083

>NTDB_id=895796 R4704_RS04545 WP_000249556.1 854994..855935(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 11012]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=895796 R4704_RS04545 WP_000249556.1 854994..855935(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 11012]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAAAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364