Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   R4704_RS04035 Genome accession   NZ_CP137109
Coordinates   772589..773413 (-) Length   274 a.a.
NCBI ID   WP_001036786.1    Uniprot ID   A0A0B7LDF1
Organism   Streptococcus pneumoniae strain 11012     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 767589..778413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4704_RS04030 - 771041..772372 (-) 1332 WP_000390042.1 hemolysin family protein -
  R4704_RS04035 endA 772589..773413 (-) 825 WP_001036786.1 DNA-entry nuclease EndA Machinery gene
  R4704_RS04040 - 773452..773640 (-) 189 WP_001813390.1 DNA-directed RNA polymerase subunit beta -
  R4704_RS04045 murA 773633..774916 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  R4704_RS04050 - 774940..775170 (-) 231 WP_000250384.1 DUF1146 family protein -
  R4704_RS04055 sepM 775228..776265 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  R4704_RS04060 coaD 776249..776737 (-) 489 WP_001280746.1 pantetheine-phosphate adenylyltransferase -
  R4704_RS04065 rsmD 776727..777266 (-) 540 WP_000706957.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  R4704_RS04070 asnA 777331..778323 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29921.59 Da        Isoelectric Point: 10.1197

>NTDB_id=895784 R4704_RS04035 WP_001036786.1 772589..773413(-) (endA) [Streptococcus pneumoniae strain 11012]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=895784 R4704_RS04035 WP_001036786.1 772589..773413(-) (endA) [Streptococcus pneumoniae strain 11012]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCTCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7LDF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

99.635

100

0.996
  endA Streptococcus pneumoniae D39

99.635

100

0.996
  endA Streptococcus pneumoniae R6

99.635

100

0.996
  endA Streptococcus pneumoniae TIGR4

99.635

100

0.996