Detailed information NTG0089547

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: LDO07_RS00105
• Genome accession: NZ_AP024937
• Coordinates: 20118..22781 (-)
• Length: 887 a.a.
• NCBI ID: WP_223965811.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain AK1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 15118..27781

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDO07_RS00085 (TthAK1_00140)	-	15964..16596 (-)	633	WP_143584081.1	histidine phosphatase family protein	-
LDO07_RS00090 (TthAK1_00150)	purM	16593..17594 (-)	1002	WP_143584083.1	phosphoribosylformylglycinamidine cyclo-ligase	-
LDO07_RS00095 (TthAK1_00160)	gatB	17600..19009 (-)	1410	WP_143584085.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
LDO07_RS00100 (TthAK1_00170)	pilT	19020..20105 (-)	1086	WP_143584087.1	type IV pilus twitching motility protein PilT	Machinery gene
LDO07_RS00105 (TthAK1_00180)	pilF	20118..22781 (-)	2664	WP_223965811.1	type IV pilus assembly ATPase PilB	Machinery gene
LDO07_RS00110 (TthAK1_00190)	-	22778..23263 (-)	486	WP_014628818.1	YqeG family HAD IIIA-type phosphatase	-
LDO07_RS00115 (TthAK1_00200)	pgeF	23298..24026 (-)	729	WP_014628819.1	peptidoglycan editing factor PgeF	-
LDO07_RS00120 (TthAK1_00210)	surE	24023..24778 (-)	756	WP_014628820.1	5'/3'-nucleotidase SurE	-
LDO07_RS00125 (TthAK1_00220)	-	24837..25043 (-)	207	WP_143584091.1	cold-shock protein	-
LDO07_RS00130 (TthAK1_00230)	-	25145..25792 (-)	648	WP_223966705.1	transposase	-
LDO07_RS00135	-	25734..25919 (+)	186	WP_223966770.1	hypothetical protein	-
LDO07_RS00140 (TthAK1_00250)	-	26237..27526 (+)	1290	WP_014628822.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97928.05 Da
• Isoelectric Point: 5.5424

>NTDB_id=89547 LDO07_RS00105 WP_223965811.1 20118..22781(-) (pilF) [Thermus thermophilus strain AK1]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLMLGEFLLKKGLISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAALLL
RSDALRYGAVPIGFQNGKVEVVLSDPRHKEAVAQLLNRPARFYLALPQAWEELFRRAYPQKNRLGEVLVQEGKLSREALK
EALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRRGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYVDPEEDPPD
PGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNHEVAPAVATEAAITKLIERFYGKAELS
EIAKEFAKKQEEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVISV
VKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKPYG
IFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRDS
ETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGLSE
AEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTLEE
VLARTIE

Nucleotide

• Length: 2664 bp

>NTDB_id=89547 LDO07_RS00105 WP_223965811.1 20118..22781(-) (pilF) [Thermus thermophilus strain AK1]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAGAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTGGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTGTACGCCCTGGCCAAACACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAGAAGGACCTCATGCTCGGGGAGTTCCTCCTGAAAAAGGGGCTGATCTCCCGCGAGGCCCTGGAGGAGGCCCT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CCTTGGCGGAGCAGAAGGGGCTAGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCCGACCCCTCCGCCGCCCTCCTCCTC
CGCTCCGACGCCCTGCGCTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGAAGGTGGAGGTGGTCCTCTCCGACCCCCG
GCACAAGGAAGCGGTGGCCCAGCTCCTCAATCGGCCTGCCCGCTTCTACCTCGCCCTCCCCCAGGCCTGGGAGGAGCTCT
TCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAGGCGCTAAAG
GAAGCCCTGGAGGTGCAGAAGGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCGCCC
CGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCGGGGCGGGGGCCGGCTGGAGGACACGCTGGTCCAGTCGGGGAAGC
TCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGGAGGACCCCCCCGAT
CCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCGCCT
CGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGAACC
ACGAGGTGGCCCCCGCTGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTCTCG
GAGATCGCCAAGGAGTTCGCCAAAAAGCAGGAGGAGGAGGCCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGCCCAGAA
GTTCGTGAAGCAGGTGATCCGGGAGGCCTTCCTCCAGGACGCCTCCGACATCCACATCGAGCCCAGGCAGAACGACGTCC
AGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATCTCCGTG
GTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAACGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGGGGCCAT
CGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGGCCTCGG
ACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCCTACGGC
ATCTTCCTCATCACCGGGCCCACAGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCCCGA
CAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCGCAGGCGG
GCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGACTCC
GAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGCCGCCCA
GGCCATCACCCGCCTGGACGAGATGGGGGTGGAACCCTTCAACATCTCCGCCGCCCTCATCGGCGTCCTCTCCCAGCGCC
TGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTTTCCGAG
GCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCGCTACGC
CATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCAAGGAGA
TCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTCGAGGAG
GTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (511-777)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-301)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-478)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	98.875	100	0.991
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	55.955	100	0.561